Products can appear in non-template control (NTC) wells due to the ability of some primer sequences to form structures that can be used as substrates by DNA polymerases. The most common […]
Daily Archives: September 12, 2020
Why is my no template control (NTC) real-time Ct value < 35 cycles in my qPCR Assay?
There is DNA contamination somewhere in your PCR assay system. Use only PCR-grade reagents and lab ware. Wear gloves throughout the procedure. Always use fresh pipette tips, water and other […]
qPCR: Avoiding Signals in the No-template Control (NTC)
This month’s question from the Molecular Biology Forums (online at molecularbiology.forums.biotechniques.com) comes from the “Real-time qPCR/qRT-PCR Methods” section. Entries have been edited for concision and clarity. Mentions of specific products […]