Product
Category: Pre-made recombinant AAV; AAV-CMV-FLPo; AAV-FLPo;
AAV(PHP.eB)-CMV-FLPo; AAV(PHP.eB)-FLPo
Description: FLP
originally identified from Saccharomyces cerevisiae is a site-specific
recombinases (SSR) from the φ integrase family and recognizes distinct 34 bp
FRT sequence. It has been widely used for generating conditional knockout
mice, mediated by FLP/FRT system. Initial use of FLP in mammalian cells
revealed inefficient recombinase activity due to thermo-instability of the FLP
protein. Subsequent screening for thermo-stable mutants resulted in the
identification of FLPe which was 4-fold increase in recombination efficiency
at 37 °C. However the recombination efficiency of FLPe in cells remains very
low because of its non-mammalian origin.
FLPo is a mouse codon optimized FLPe that greatly increases the FRT
recombination efficiency for both in vivo and in vitro in mouse. This AAV(PHP.eB)-CMV-FLPo
pre-packaged rAAV in serotype PHP.eB (with capsid from AAV serotype PHP.eB and 2xITR
from AAV serotype 2) will over-express
FLPo recombinase under the CMV
promoter. Ready
to use format.
Titer: >
1x10^13 VG/mL
Size: > 30 µL
(Std Pack)
Storage
Buffer: Sterile
PBS
Infection Protocol: ![](http://www.signagen.com/images/pdficon.gif)
Vector
Map:
![Pre-made recombinant AAV; AAV-CMV-FLPo; AAV-FLPo; AAV(PHP.eB)-CMV-FLPo; AAV(PHP.eB)-FLPo](http://signagen.com/AAV_Vector_Maps/AAV-CMV-FLPo.png)