Abstract The targeting efficiency of knockin sequences via homologous recombination (HR) is generally low. Here we describe a method we call Tild-CRISPR (targeted integration with linearized dsDNA-CRISPR), a targeting strategy […]
CRISPR Genome Editing in Mice from He Lab at UC Berkeley
CRISPR-mediated mouse genome editing is typically accomplished by microinjection of Cas9 DNA/RNA and sgRNA into zygotes to achieve editing in one step. We developed a simple and economic electroporation-based strategy, […]
Validated GPAM Target Region Sequence
Validated GPAM Target Region Sequence: GACGGAGGCTCGATGAAAC GACTGTGGGTTCAAACCTA
Validated Tbk1 Target Region Sequence
Validated Tbk1 Target Region Sequence: GCAGAGCACTTCCAACCATCT
Several Validated siRNA
siRNA Species Target sequence PPARα mouse 5´-GGGUCAUACUCGCGGGAAAdtdt-3´ PPARα mouse 5´-GGCUGAAGCUGGUGUACGAdtdt-3´ PPARα mouse 5´-GGGCAAGAGAAUCCACGAAdtdt-3´ PPARα mouse 5´-ACACAAUGCAAUUCGCUUUdtdt-3´ GYK […]
Argininosuccinate Lyase (ALS) Validated Target Region
CACCTTCAAACTGAACTCCAA AGGAGGCTGCTGTGTGTTT
Validated gpNaV1.7 shRNA
GGGCATAGATTACGTGAAATTCAAGAGATTTCACGTAATCTATGCCC
Validated Target Region for Mouse STX18
ACGACTAACTGGTGAATG
shRNA/siRNA Design Online Tools
http://cancan.cshl.edu/RNAi_central/RNAi.cgi?type=shRNA https://www.invivogen.com/sirnawizard/ https://rnaidesigner.thermofisher.com/rnaiexpress/