Miscellaneous - 17. page

1) Prepare 6 ml of 5X ISO Buffer in a 15 ml falcon tube as follows: 3 ml 1 M Tris-HCl pH 7.5 + 150µl 2 M MgCl2 + 240 µl 100 […]

Gene specific primers: GSS-F: SbfI Kozak GSS AGGCT cctgcagg CCACCATG NNNNNNNNNNNNNNNNNNNNN GSS-R: GSS * Xho1 (NNNNNNNNNNNNNNNNNNNNN TAG ctcgag ACCCAGCTTTC) For example: GAAAGCTGGGT ctcgag CTA N(15–25)   Universal Primers attL1-F ccccGATGAGCAATGCTTTTTTATAATGCCAACTTTGTACAAAAAAGCA […]

1.Excision reaction. 44.5 mM Tris–borate (pH 8.3) 7.5 mM Tris–HCl (pH 8.0) 1.15 mM EDTA 5 mM spermidine 60 mM KCl 0.275 mg/ml BSA 15% (v/v) glycerol. Int 50~100 nM […]