Miscellaneous

The OmpA signal peptide is a 21-amino acid sequence that targets fused proteins to the Sec secretion pathway in E. coli. It can be used to increase protein yield and simplify purification […]

High-cell-density cultivation (HCDC) is a process that improves the formation of microbial biomass and products. It can be used for microorganisms such as bacteria, archaea, and yeasts. HCDC can make the fermentation process […]

To protect RNA from degradation, you can:  RNA is prone to enzymatic degradation and hydrolysis. Even at low temperatures, RNA can still be reactive, and some ribozymes can be enhanced by […]

Unlike DNA, RNA is usually single-stranded. Additionally, RNA contains ribose sugars rather than deoxyribose sugars, which makes RNA more unstable and more prone to degradation. RNA is generally stable at –80° […]

Conclusion In conclusion, the absence of apparent supercoiling in plasmid DNA preparations can result from a variety of factors related to DNA extraction, purification, handling, and analysis. By systematically addressing […]

Successful transient transfection relies on many factors, starting with a successful plasmid prep. To ensure high transfection efficiency, resulting DNA from your plasmid preps must be transfection grade. But what […]

When uncut plasmid DNA is isolated and run on an agarose gel, you may observe two, three, or even four or more bands. Hopefully, the majority of your isolated DNA […]

For a 20µl reaction: Component   Volume (µl)   Final Concentration H2O 12.9   10x homemade core buffer 2 1x 2mM dNTP mix 2 0.2mM 20µM primer mix 0.5 500nM […]

Reagent Volume (for 150 µL) qPCR mix (2×) 100 µL Sense primer (1 µM) 2 µL Antisense primer (1 µM) 2 µL Fluorescein (1 µM) 2 µL SYBR Green (40×) […]

As DNA is a highly hydrophilic molecule, it usually cannot pass through the cell membrane of bacteria. Hence, to make bacteria capable of internalizing genetic material, they must be made […]