LV-ChAT-GFP-WPRE Lentivirus Ready to Package
Monthly Archives: April 2016
Several Published siRNAs
iRNA sequences JAK1 #1 CCACAUAGCUGAUCUGAAA JAK1 #2 GACAUGAUAUUGAGAACGA JAK1 #3 UUACAAGGAUGACGAAGGA JAK1 #4 CGGAUGAGGUUCUAUUUCA JAK2 SP GAGCAAAGAUCCAAGACUA GCCAGAAACUUGAAACUUA GAUCCUGGCAUUAGUAUUA ACAGAAUGCUGGAACAAUA TYK2 SP GCAUCCACAUUGCACAUAA UCAAAUACCUAGCCACACU CAAUCUUGCUGACGUCUUG CACAAGGACCAACGUGUAU JAK3 SP GCGCCUAUCUUUCUCCUUU CCAGAAAUCGUAGACAUUA […]
A Validated siRNA Targeting Mouse/Rat RGS4/5
RGS5 : CAGACUCUGCUGUUGACCUUGUCAU GGUGAACAUUGACCACUUCACUAAA RGS4: AAAGCUGCCAGUCCACAUUCAUGGU
Overview of Lentivirus Pseudotypes
Overview of Lentivirus Pseudotypes Family Genus Species Vector References Rhabdoviridae Vesiculovirus Vesicular stomatitis virus (Indiana virus) HIV-1 [Akkina, 1996][Naldini, 1996][Reiser, 1996] HIV-2 [Poeschla, 1998a] FIV [Poeschla, 1998b] EIAV [Olsen, 1998] […]
LV-HB9-Citrine-WPRE Lentivirus Ready to Package
LV-HB9-Citrine-WPRE Lentivirus Ready to Package
A universal DNA qPCR based approach for lentivirus titration
A quantitative, DNA-based, real-time PCR approach to determine the number of lentivirus particles that are present in vector preparations was reported. In this approach, the minus strong-stop cDNA fragment that is present in viral capsids serves as […]
Primers targeting minus strong-stop cDNA fragment for lentivirus titration
Forward: 5′-AGCTTGCCTTGAGTGCTTCA-3′ Reverse: 5′-TGACTAAAAGGGTCTGAGGGA-3′
Optimal MOIs for Some Cells Infected with Lentivirus
Cell line Tissue Cancer/cell type Species MOI A431 Epithelial Carcinoma Human 5 A549 Lung Carcinoma Human 5 Astrocytes Nervous system Primary Human 1 B16-F10 Epithelial Melanoma, metastatic Mouse 5 BMM Bone Marrow Primary Human 8 […]