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Monthly Archives: January 2016

Full Sequence of the Inducible Caspase 9 (iCasp9)

atgctcgagggagtgcaggtggaGacTatctccccaggagacgggcgcaccttccccaagcgcggccagacctgcgtggtgcactacaccgggatgctt gaagatggaaagaaagttgattcctcccgggacagaaacaagccctttaagtttatgctaggcaagcaggaggtgatccgaggctgggaagaaggg gttgcccagatgagtgtgggtcagagagccaaactgactatatctccagattatgcctatggtgccactgggcacccaggcatcatcccaccacatgccact ctcgtcttcgatgtggagcttctaaaactggaatctggcggtggatccggagtcgacggatttggtgatgtcggtgctcttgagagtttgaggggaaatg cagatttggcttacatcctgagcatggagccctgtggccactgcctcattatcaacaatgtgaacttctgccgtgagtccgggctccgcacccgcactggct ccaacatcgactgtgagaagttgcggcgtcgcttctcctcgctgcatttcatggtggaggtgaagggcgacctgactgccaagaaaatggtgctggcttt gctggagctggcgcGgcaggaccacggtgctctggactgctgcgtggtggtcattctctctcacggctgtcaggccagccacctgcagttcccaggggct gtctacggcacagatggatgccctgtgtcggtcgagaagattgtgaacatcttcaatgggaccagctgccccagcctgggagggaagcccaagctcttt ttcatccaggcctgtggtggggagcagaaagaccatgggtttgaggtggcctccacttcccctgaagacgagtcccctggcagtaaccccgagccagat gccaccccgttccaggaaggtttgaggaccttcgaccagctggacgccatatctagtttgcccacacccagtgacatctttgtgtcctactctactttcccag gttttgtttcctggagggaccccaagagtggctcctggtacgttgagaccctggacgacatctttgagcagtgggctcactctgaagacctgcagtccctc ctgcttagggtcgctaatgctgtttcggtgaaagggatttataaacagatgcctggttgctttaatttcctccggaaaaaacttttctttaaaacatcagtcg actatccgtacgacgtaccagactacgcactcgactaa

Common Epitope Tags

Common Epitope Tags Tag Protein Sequence DNA Sequence* FLAG DYKDDDDK GAC TAC AAA GAC GAT GAC GAC AAG HA YPYDVPDYA TAC CCA TAC GAT GTT CCA GAT TAC GCT His […]

pHuji, a pH-sensitive red fluorescent protein for imaging of exo- and endocytosis.

atggtgagcaagggcgaggagaataacatggccatcatcaaggagttcatgcgcttcaaggtgcacatggagggctccgtgaacggcca cgagttcgagatcgagggcgagggcgagggccgcccctacgaggcctttcagaccgctaagctgaaggtgaccaagggtggccccctgcc cttcgcctgggacatcctgtcccctcagttcatgtacggctccaaggtctacattaagcacccagccgacatccccgactacttcaagctgtcct tccccgagggcttcaggtgggagcgcgtgatgaacttcgaggacggcggcattattcacgttaaccaggactcctccctgcaggacggcgt gttcatctacaaggtgaagctgcgcggcaccaacttcccctccgacggccccgtaatgcagaagaagaccatgggctgggaggcctccgag gagcggatgtaccccgaggacggcgccctgaagagcgagattaagtataggctgaagctgaaggacggcggccactacgccgccgaggt caagaccacctacaaggccaagaagcccgtgcagctgcccggcgcctacatcgtggacatcaagttggacatcgtgtcccacaacgaggacta caccatcgtggaacagtacgaacgcgccgagggccgccactccaccggcggcatggacgagctgtacaagGctagCTAA

mEos3.2

Monomeric variant of green-to-red photoswitchable fluorescent protein EosFP, engineered for reduced aggregation relative to mEos2. ATGAGTGCGATTAAGCCAGACATGAAGATCAAACTCCGTATGGAAGGCAACGTAAACGGGCACCACTTTGTGATCGACGGAGATGGTACAGGCAAGCC TTTTGAGGGAAAACAGAGTATGGATCTTGAAGTCAAAGAGGGCGGACCTCTGCCTTTTGCCTTTGATATCCTGACCACTGCATTCCATTACGGCAACAGG GTATTCGCCAAATATCCAGACAACATACAAGACTATTTTAAGCAGTCGTTTCCTAAGGGGTATTCGTGGGAACGAAGCTTGACTTTCGAAGACGGGGGC ATTTGCAACGCCAGAAACGACATAACAATGGAAGGGGACACTTTCTATAATAAAGTTCGATTTTATGGTACCAACTTTCCCGCCAATGGTCCAGTTATGC AGAAGAAGACGCTGAAATGGGAGCCCTCCACTGAGAAAATGTATGTGCGTGATGGAGTGCTGACGGGTGATATTGAGATGGCTTTGTTGCTTGAAGGA AATGCCCATTACCGATGTGACTTCAGAACTACTTACAAAGCTAAGGAGAAGGGTGTCAAGTTACCAGGCGCCCACTTTGTGGACCACTGCATTGAGATT TTAAGCCATGACAAAGATTACAACAAGGTTAAGCTGTATGAGCATGCTGTTGCTCATTCTGGATTGCCTGACAATGCCAGACGATAA