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Monthly Archives: February 2016

hAsCpf1-NLS-3xHA Sequence

Full Sequence of hAsCpf1-NLS-3xHA Sequence: ATGACACAGTTCGAGGGCTTTACCAACCTGTATCAGGTGAGCAAGACACTGCGGTTTGAGCTGATCCCACAGGGCAAGACCCTGAAGCACATCCAGGAGCAGGGCT TCATCGAGGAGGACAAGGCCCGCAATGATCACTACAAGGAGCTGAAGCCCATCATCGATCGGATCTACAAGACCTATGCCGACCAGTGCCTGCAGCTGGTGCAGCT GGATTGGGAGAACCTGAGCGCCGCCATCGACTCCTATAGAAAGGAGAAAACCGAGGAGACAAGGAACGCCCTGATCGAGGAGCAGGCCACATATCGCAATGCCA TCCACGACTACTTCATCGGCCGGACAGACAACCTGACCGATGCCATCAATAAGAGACACGCCGAGATCTACAAGGGCCTGTTCAAGGCCGAGCTGTTTAATGGCAA GGTGCTGAAGCAGCTGGGCACCGTGACCACAACCGAGCACGAGAACGCCCTGCTGCGGAGCTTCGACAAGTTTACAACCTACTTCTCCGGCTTTTATGAGAACAGG AAGAACGTGTTCAGCGCCGAGGATATCAGCACAGCCATCCCACACCGCATCGTGCAGGACAACTTCCCCAAGTTTAAGGAGAATTGTCACATCTTCACACGCCTGA TCACCGCCGTGCCCAGCCTGCGGGAGCACTTTGAGAACGTGAAGAAGGCCATCGGCATCTTCGTGAGCACCTCCATCGAGGAGGTGTTTTCCTTCCCTTTTTATAAC CAGCTGCTGACACAGACCCAGATCGACCTGTATAACCAGCTGCTGGGAGGAATCTCTCGGGAGGCAGGCACCGAGAAGATCAAGGGCCTGAACGAGGTGCTGAAT CTGGCCATCCAGAAGAATGATGAGACAGCCCACATCATCGCCTCCCTGCCACACAGATTCATCCCCCTGTTTAAGCAGATCCTGTCCGATAGGAACACCCTGTCTTT CATCCTGGAGGAGTTTAAGAGCGACGAGGAAGTGATCCAGTCCTTCTGCAAGTACAAGACACTGCTGAGAAACGAGAACGTGCTGGAGACAGCCGAGGCCCTGTT TAACGAGCTGAACAGCATCGACCTGACACACATCTTCATCAGCCACAAGAAGCTGGAGACAATCAGCAGCGCCCTGTGCGACCACTGGGATACACTGAGGAATGC CCTGTATGAGCGGAGAATCTCCGAGCTGACAGGCAAGATCACCAAGTCTGCCAAGGAGAAGGTGCAGCGCAGCCTGAAGCACGAGGATATCAACCTGCAGGAGA TCATCTCTGCCGCAGGCAAGGAGCTGAGCGAGGCCTTCAAGCAGAAAACCAGCGAGATCCTGTCCCACGCACACGCCGCCCTGGATCAGCCACTGCCTACAACCC TGAAGAAGCAGGAGGAGAAGGAGATCCTGAAGTCTCAGCTGGACAGCCTGCTGGGCCTGTACCACCTGCTGGACTGGTTTGCCGTGGATGAGTCCAACGAGGTG GACCCCGAGTTCTCTGCCCGGCTGACCGGCATCAAGCTGGAGATGGAGCCTTCTCTGAGCTTCTACAACAAGGCCAGAAATTATGCCACCAAGAAGCCCTACTCCG TGGAGAAGTTCAAGCTGAACTTTCAGATGCCTACACTGGCCTCTGGCTGGGACGTGAATAAGGAGAAGAACAATGGCGCCATCCTGTTTGTGAAGAACGGCCTGT ACTATCTGGGCATCATGCCAAAGCAGAAGGGCAGGTATAAGGCCCTGAGCTTCGAGCCCACAGAGAAAACCAGCGAGGGCTTTGATAAGATGTACTATGACTACT TCCCTGATGCCGCCAAGATGATCCCAAAGTGCAGCACCCAGCTGAAGGCCGTGACAGCCCACTTTCAGACCCACACAACCCCCATCCTGCTGTCCAACAATTTCATC GAGCCTCTGGAGATCACAAAGGAGATCTACGACCTGAACAATCCTGAGAAGGAGCCAAAGAAGTTTCAGACAGCCTACGCCAAGAAAACCGGCGACCAGAAGGGC TACAGAGAGGCCCTGTGCAAGTGGATCGACTTCACAAGGGATTTTCTGTCCAAGTATACCAAGACAACCTCTATCGATCTGTCTAGCCTGCGGCCATCCTCTCAGTA TAAGGACCTGGGCGAGTACTATGCCGAGCTGAATCCCCTGCTGTACCACATCAGCTTCCAGAGAATCGCCGAGAAGGAGATCATGGATGCCGTGGAGACAGGCAA GCTGTACCTGTTCCAGATCTATAACAAGGACTTTGCCAAGGGCCACCACGGCAAGCCTAATCTGCACACACTGTATTGGACCGGCCTGTTTTCTCCAGAGAACCTGG CCAAGACAAGCATCAAGCTGAATGGCCAGGCCGAGCTGTTCTACCGCCCTAAGTCCAGGATGAAGAGGATGGCACACCGGCTGGGAGAGAAGATGCTGAACAAGA AGCTGAAGGATCAGAAAACCCCAATCCCCGACACCCTGTACCAGGAGCTGTACGACTATGTGAATCACAGACTGTCCCACGACCTGTCTGATGAGGCCAGGGCCCT GCTGCCCAACGTGATCACCAAGGAGGTGTCTCACGAGATCATCAAGGATAGGCGCTTTACCAGCGACAAGTTCTTTTTCCACGTGCCTATCACACTGAACTATCAGG […]

Several Validated siRNAs

PLEKHM1 (cDNA) # 1 GCAAAGUCCUGGCAUCCUA # 2 GAACGCGUCUCUGUACGAG VPS11 (cDNA) GAAACGAGAUCUCCAUGAU VPS41 (cDNA) GAGAAUGAAUGUAGAGAU RAB7A (cDNA) # 1 GUACAAAGCCACAAUAGGA # 2 AAACGGAGGUGGAGCUGUA

Gln tRNA Promoter

GGTTCCATGGTGTAATGGTTAGCACTCTGGACTCTGAATCCAGCGATCCGAGTTCAAATCTCGGTGGAACCT

Several Validated siRNAs

Mouse Rab9-siRNA sense, CGGCGACUAUCCUUACUUUTT and antisense, AAAGUAAGGAUAGUCGCCGTT ; mouse Beclin 1-siRNA sense, GAGUUGCCGUUAUACUGUUCU and antisense, AACAGUAUAACGGCAACUCCU ; mouse LAMP-2A-siRNA sense, GAGGAGUACUUAUUCUAGUGU and antisense, ACUAGAAUAAGUACUCCUCCC ; mouse Hsc70-siRNA sense, CGAUGAAGCUGUUGCCUAUTT and antisense, AUAGGCAACAGCUUCAUCGGG; […]

Full Sequence of mt-mKeima

ATGCATCTCGAGGCCACCATGTCCGTCCTGACGCCGCTGCTGCTGCGGGGCTTGACAGGCTCGGCCCGGCGGCTCCCAGTGCCGCGCGCCAAGATCCAT TCGTTGCCGCCGGAGGGGAAGCTTGGGATGTCCGTCCTGACGCCGCTGCTGCTGCGGGGCTTGACAGGCTCGGCCCGGCGGCTCCCAGTGCCGCGCGCC AAGATCCATTCGTTGCCGCCGGAGGGGAAGCTCGGGggatccGCCATGGTGAGCGTGATCGCCAAGCAGATGACCTACAAGGTGTACATGAGCGGCACC GTGAACGGCCACTACTTCGAGGTGGAGGGCGACGGCAAGGGCAAGCCCTACGAGGGCGAGCAGACCGTGAAGCTGACCGTGACCAAGGGTGGCCCCC TGCCCTTCGCCTGGGACATCCTGAGCCCCCAGCTCCAGTACGGCAGCATCCCCTTCACCAAGTACCCCGAGGACATCCCCGACTACTTCAAGCAGAGCTT CCCCGAGGGCTACACCTGGGAGCGCAGCATGAACTTCGAGGACGGCGCCGTGTGCACCGTGAGCAACGACAGCAGCATCCAGGGCAACTGCTTCATCT ACAACGTGAAGATCAGCGGCGAGAACTTCCCCCCCAACGGCCCCGTGATGCAGAAGAAGACCCAGGGCTGGGAGCCCAGCACCGAGCGCCTGTTCGC CCGCGACGGAATGCTGATCGGCAACGACTACATGGCCCTGAAGCTGGAGGGCGGCGGCCACTACCTGTGCGAGTTCAAGAGCACCTACAAGGCCAAGA AGCCCGTGAGGATGCCCGGCCGCCACGAGATCGACCGCAAGCTGGACGTGACCAGCCACAACCGCGACTACACCAGCGTGGAGCAGTGCGAGATCGCC ATCGCCCGCCACTCCCTGCTGGGCTAA

siRNAs targeting human Caspase

Human Caspase-1 target region: AAGGGGCACAGGCATGCCAAA Human Apaf-1 target region: : AATTGGTGCACTTTTACGTGA Human Caspase-2 target region:  AAACAGCTGTTGTTGAGCGA