Ad.MAX™ Technology for Maximum Adenovirus Production: Ad.MAX™ technology was developed by genetically modifying virus packaging cell, HEK293 cell and adenoviral shuttle vector or adenoviral genome for maximum adenovirus production. The […]
Primers for Lentivirus Titration using qRT-PCR
Vector and region amplified Primers and probes dedicated for the amplification of lentiviral or transgenic DNA sequences Primers and probes dedicated for the amplification of internal control DNAs (cellular genome […]
Fusion Cloning, Phusion Cloning, Pfusion Cloning and Confusion PCR Cloning
In-Fusion PCR Cloning: Clontech, http://www.freepatentsonline.com/7575860.html Cold Fusion Cloning Kit: SBI Fast Seamless Cloning Kit: Dogene CloneEZ Kit: Genescript GENEART Seamless Cloning and Assembly Kit: Invitrogen SLIC uses the 3′ […]
Gibson Assembly Mix for joining two or more PCR fragments
1) Prepare 6 ml of 5X ISO Buffer in a 15 ml falcon tube as follows: 3 ml 1 M Tris-HCl pH 7.5 + 150µl 2 M MgCl2 + 240 µl 100 […]
Rapid one-step LR recombinational cloning using an enzyme mixture containing Int, IHF and Xis (Gateway Clonase) and two step PCR amplification
Gene specific primers: GSS-F: SbfI Kozak GSS AGGCT cctgcagg CCACCATG NNNNNNNNNNNNNNNNNNNNN GSS-R: GSS * Xho1 (NNNNNNNNNNNNNNNNNNNNN TAG ctcgag ACCCAGCTTTC) For example: GAAAGCTGGGT ctcgag CTA N(15–25) Universal Primers attL1-F ccccGATGAGCAATGCTTTTTTATAATGCCAACTTTGTACAAAAAAGCA […]
Home-made Gateway Clonase II
1.Excision reaction. 44.5 mM Tris–borate (pH 8.3) 7.5 mM Tris–HCl (pH 8.0) 1.15 mM EDTA 5 mM spermidine 60 mM KCl 0.275 mg/ml BSA 15% (v/v) glycerol. Int 50~100 nM […]
SignaGen’s In Vitro siRNA Transfection Reagents
siRNA Transfection Reagent Selection Chart. Reagents Features Transfection Type Toxicity PepMute™ – Exceptional good for siRNA transfection – OK for DNA/siRNA co-transfection siRNA (Yes) DNA/siRNA (No) ++ GenMute™ – Exceptional […]
>300 Pre-made Ready to Use Adenoviruses from SignaGen
SiganGen is offering >300 pre-made and ready to use adenovirus vectors. All the pre-packaged adenoviruses were packaged from Ad.MAX™ system with a serotype 5 adenoviral backbone* (E1/E3 deletion). All the […]
SignaGen’s rAAV Production Service
Multi Approaches to Produce Super Infectious rAAV Particles at Super High Titer: Not like adenovirus, it is relatively harder to achieve high yield of rAAV with regular rAAV production procedures. […]
SignaGen’s In Vitro DNA Transfection Reagents
Transfection Reagent Selection Chart. Reagents Features Transfection Type Toxicity* CalFectin™ – Traditional recipe with new formulation – Most affordable and efficient – Best for large scale virus, antibody and protein […]