CMV Promoter: CMV-F TTC CTA CTT GGC AGT ACA TCT ACG CMV-R GTC AAT GGG GTG GAG ACT TGG CMV-P TGA GTC AAA CCG CTA TCC ACG CCC A AAV2 […]
Monthly Archives: October 2019
Commonly Used Primers
3’AOX1 GCAAATGGCATTCTGACATCC For Pichia vectors with AOX1 terminator, reverse primer 5’AOX1 GACTGGTTCCAATTGACAAGC For Pichia vectors with AOX1 promoter, forward primer 35S promoter CTATCCTTCGCAAGACCCTTC CaMV 35S promoter, forward primer AC5 ACACAAAGCCGCTCCATCAG […]
Sim1 Specific Promoter for PVN Neurons
1 GGTACCGAGG CGCGCAGCCG TGGGGAAATC CTGGCCACTG TCTGGGATGC 51 TATTCGCACG CGTTCCTGGA GCCTCGGCTA GCCAGCCCCC GCAAGGCCGC 101 ACGCTGTGCG CAGACACCCG AGTCTAGGGA GCGCGAGTGT CACTCGGCAC 151 GCCGAAGACC TCGGGGTTTG GAGCGGCGGC GGCAGCGGGG CAGCGGCGAC 201 CCCGCGCCCG GGGCTCATTA GGAGCGGAGC TGCCAGGCTC ACTGTAGGAC […]
Wt AAV P5 Promoter for Rep
ggaggggtggagtcgtgacgtgaattacgtcatagggttagggaggtcctgtattagaggtcacgtgagtgttttgcgacattttgcgacaccatgtggtcacgctgggtatttaagcccg agtgagcacgcagggtctccattttgaagcgggaggtttgaac
Validated Target Region against Mouse Slc16a7 (MCT2) Gene
CATTGGAGGTTTAGGATTA
Validated Target Region against Mouse SCN3A
GTTCGACCCTGACGCCACC
rcAAV Assay via PCR
PCR and sequencing. The following primers were used for PCR amplification for analysis of rcAAV: D region, 5′-CTCCATCACTAGGGGTTCC-3′ (AAV2 nt 126 to 144); rep, 5′-GGCAGATGCCCGTCAAGGT-3′ (AAV2 nt 379 to 361); and cap, 5′-CAGAGATGTGTACCTTCAG-3′ […]