At SignaGen Laboratories, Lentivector Design & Synthesis is powered by software-based in silico design to ensure accuracy, consistency, and predictable control of gene expression before any DNA is built. Our team digitally assembles promoters, regulatory elements, payloads, and safety features, then performs sequence-level checks to eliminate frame errors, unwanted restriction sites, recombination-prone regions, and incompatibilities that can reduce viral yield or expression performance.

We support the generation of lentiviral vectors for transgene overexpression as well as multiple RNA-based gene regulation strategies within lentiviral transfer vectors, including systems designed to overexpress:

• A transgene that is obtained via gene synthesis, followed by verification by WPS of the NanoPore approach
• shRNA for robust gene silencing under Pol III promoters
• shRNAmir for tissue-specific or regulated gene silencing under Pol II promoters
• miRNA for endogenous microRNA-based gene expression modulation
• gRNA for CRISPR-directed gene editing or transcriptional regulation
• TuD (Tough Decoy) RNA for efficient knockdown of specific miRNAs

Once finalized, each digital blueprint is translated into high-fidelity DNA through synthesis and cloning, followed by verification to confirm that the physical construct precisely matches the approved in silico design—delivering reliable, reproducible lentivectors ready for downstream lentivirus production and functional studies.

Please contact us at info@signagen.com for more details on our lentiviral vector design and synthesis