All AAV packaging at SignaGen Laboratories is performed using a helper-free system in HEK293T cells to maximize safety, efficiency, and consistency, eliminating the need for replication-competent helper viruses. The process begins with triple plasmid transfection, combining the AAV cis plasmid, a packaging plasmid encoding rep and cap genes, and a helper plasmid providing adenoviral functions. Following transfection, cells are cultured under optimized conditions to support high-yield vector production. Vectors are then harvested from both the cell lysate and culture supernatant, followed by clarification and purification to produce high-titer, high-quality AAV suitable for downstream applications. This workflow ensures reproducible vector performance, scalability, and compatibility with in vivo studies.

Please note that we do not perform AAV packaging in insect cell systems; all production is exclusively in HEK293T cells. To learn more, please contact us at info@signagen.com