All AAV vector cloning at SignaGen Laboratories is performed in-house, ensuring precise control and quality at every step. AAV cis constructs are generated using versatile cloning strategies, including traditional restriction digestion followed by T4 ligase ligation, Gibson Assembly, or Golden Gate Assembly, selected based on vector design and sequence complexity. Following cloning, constructs undergo comprehensive verification using Whole Plasmid Sequencing (WPS) via Nanopore technology, providing full plasmid coverage and accurate detection of any sequence variants or rearrangements. This integrated approach ensures that all AAV vectors are correctly assembled, thoroughly validated, and ready for reliable downstream packaging and experimental use.

To learn more, please contact us at info@signagen.com