Recombinant AAV vectors are purified via ultracentrifugation to achieve high purity and consistency, making them suitable for in vivo applications. Vector genome titers are quantified using two complementary qPCR chemistries—probe-based and SYBR Green–based—both calibrated with ATCC reference materials to ensure accurate titer normalization and batch-to-batch reproducibility. To support in vivo–grade production, endotoxin levels are measured using the Limulus Amebocyte Lysate (LAL) assay, meeting stringent safety specifications. Optional advanced analyses include Mycoplasma testing and empty/full capsid quantification using a Refeyn mass photometer, providing comprehensive characterization of vector composition and overall product quality.