Service Quote Request
Please fill out the form below with your detailed inquiry.
Summary Table: Adipose-Specific Promoters & Sequence Sizes
| Promoter | Best AAV Version | Optimized Size | Target | AAV Serotype Note |
| mAdipoq | Truncated Adipoq | ~2.1 kb | Mature WAT/BAT | Most validated for systemic AAV8 delivery. |
| hAdipoq | Human Adipo-e/p | ~1.1 kb | Mature WAT/BAT | Uses a distal enhancer + proximal promoter. Very compact. |
| aP2 (Fabp4) | mAP2.2 fragment | ~2.2 kb | Mature Adipocytes | High expression but watch for liver/macrophage leakiness. |
| Ucp1 | mini-Ucp1 | ~2.0 kb | BAT/Beige Fat | Essential for thermogenic fat studies. |
| Plin1 | Proximal Plin1 | ~1.0 kb | Mature Adipocytes | Very small; ideal if your transgene (GOI) is large (>2 kb). |
| Synthetic | Ppar$\gamma$-RE x3 | < 0.5 kb | Mature Adipocytes | Ultra-short; uses tandem repeats of binding elements. |
Critical Considerations for AAV Production
1. Managing “Leakiness” with miR-122
Even with adipose-specific promoters, AAVs (especially AAV8/9) have a high natural tropism for the liver. To ensure your gene is only in fat:
- Add miR-122 target sites: Inserting 3–4 copies of the miR-122 binding site in the 3′ UTR of your vector will cause the liver to degrade your transgene’s mRNA, effectively “shaping” the expression to be fat-exclusive.
2. Recommended Serotypes
For adipose tissue, the choice of capsid (serotype) is as important as the promoter:
- AAV8: Generally considered the gold standard for systemic (IV) delivery to both White Adipose Tissue (WAT) and Brown Adipose Tissue (BAT).
- AAV9: Also highly effective for adipose, though it has higher cardiac and CNS cross-reactivity.
- AAV-Rec2: A recently engineered synthetic serotype showing high efficiency specifically in adipocytes.
Important updates waiting for you!
Enter your email address below and subscribe to our newsletter