LV-EF1a-tdTOMATO-IRES-Puro

Product Category: Pre-made recombinant lentivirus | LV-EF1α-tdTOMATO-IRES-Puro | LV-EF1A-tdTOMATO-Puro | EF1α-tdTOMATO-Puro Lentivirus | EF1A-tdTOMATO-Puro Lentivirus

Description:
LV-EF1α-tdTomato-IRES-Puro is a pre-made lentiviral vector that drives robust expression of tdTomato under the constitutive EF1α promoter, with simultaneous puromycin resistance mediated via an internal ribosome entry site (IRES) for efficient selection of transduced cells.

tdTomato is a tandem dimeric (pseudo-monomeric) derivative of DsRed and is one of the brightest red fluorescent proteins available, exhibiting approximately six-fold greater brightness than EGFP. Due to the covalent linkage of its two subunits, tdTomato functions effectively as a monomer and is well-suited for both N- and C-terminal fusion applications. It demonstrates minimal aggregation, excellent photostability, and a rapid maturation half-time of approximately one hour at 37 °C. Its strong red fluorescence and high signal intensity make it particularly suitable for a wide range of in vitro and in vivo imaging applications.

Titer: >1E+9 TU/mL

Selection Antibiotic: Puromycin

Purity*: In vivo grade and OK for both in vitro tissue culture infection and in vivo injection

Size: 2 x 25 µL (Std Pack)

Storage Buffer: PBS

Infection Protocol:

Transfer Vector Map:

* Concentrated via PEG precipitation followed by ultra-centrifugation at 25000 rpm