LV-EF1a-iCre-T2A-GFP-Puro

Product Category: Pre-made recombinant lentivirus | LV-EF1a-iCre-P2A-GFP-Puro | LV-EF1α-iCre-P2A-GFP-Puro | LV-EF1a-iCre-P2A-GFP-IRES-Puro | LV-EF1α-iCre-P2A-GFP-IRES-Puro

Description:
LV-EF1α-iCre-T2A-GFP-Puro is a high-titer, ready-to-use lentiviral vector designed for efficient delivery and stable expression of improved Cre recombinase (iCre) in a wide range of mammalian cells. The construct is driven by the constitutive human EF1α promoter, ensuring robust and sustained transgene expression in both dividing and non-dividing cells.

The iCre coding sequence is linked to GFP via a T2A self-cleaving peptide, enabling bicistronic expression from a single transcript. This allows real-time visualization of transduced cells through GFP fluorescence while maintaining independent functional expression of iCre. A puromycin resistance cassette is included for rapid antibiotic selection and establishment of stable cell populations.

This lentiviral vector is ideal for Cre-loxP–mediated recombination studies, conditional gene knockout/activation, lineage tracing, and generation of stable cell lines requiring precise genomic modification.

Titer: >1E+9 TU/mL

Selection Antibiotic: Puromycin

Purity*: In vivo grade and OK for both in vitro tissue culture infection and in vivo injection

Size: 2 x 25 µL (Std Pack)

Storage Buffer: PBS

Infection Protocol:

Transfer Vector Map:

* Concentrated via PEG precipitation followed by ultra-centrifugation at 25000 rpm