LV-EF1a-DIO-tdTomato

Product Category: Pre-made recombinant lentivirus | LV-EF1a-DIO-tdTomato | LV-EF1α-DIO-tdTomato | DIO-tdTomato lentivirus

Description:
LV-EF1α-DIO-tdTomato is a pre-made lentivirus that will Cre dependently over-express tdTomato under EF1alpha promoter, as illustrated in the cartoon below.

tdTomato is a red fluorescent protein commonly used as a reporter in molecular and cell biology. It is derived from the Discosoma sp. red fluorescent protein (DsRed) and was engineered to improve brightness, stability, and fast maturation. In the DIO scenario, the transgene of interest is inserted in reverse orientation relative to the 5′ promoter and is flanked by oppositely oriented loxP and lox2272 sites. In the absence of Cre expression, the transgene will not be produced. In the presence of Cre expression, the transgene will be “Flip-exchanged” or FLEXed, leading to the expression of the transgene. This is due to a permanent Cre-mediated recombination/inversion of the flanked transgene. This arrangement is called DIO (double-floxed inverse ORF), Cre-ON, Flex-rev (reverse), Flex-ON/FlexON, or DIO-AAV/AAV-DIO (double-floxed inverse ORF in AAV).

Titer: >1E+9 TU/mL

Selection Antibiotic: None

Purity*: In vivo grade and OK for both in vitro tissue culture infection and in vivo injection

Size: 2 x 25 µL (Std Pack)

Storage Buffer: PBS

Infection Protocol:

Transfer Vector Map:

* Concentrated via PEG precipitation followed by ultra-centrifugation at 25000 rpm