LV-EF1α-CymR-IRES-Puro

Product Category: Pre-made recombinant lentivirus | LV-EF1a-CymR-IRES-Puro | LV-EF1α-CymR-IRES-Puro | EF1α-CymR-Puro Lentivirus | EF1A-CymR-Puro Lentivirus

Description:
LV-EF1α-CymR-IRES-Puro is a pre-made, replication-deficient lentiviral vector designed for the stable delivery and expression of the CymR repressor protein in mammalian cells. CymR is a regulatory protein derived from Pseudomonas putida that specifically binds to the cumate operator (CuO), enabling tight and reversible transcriptional control in cumate-inducible gene expression systems.

In this construct, CymR expression is driven by the human EF1α promoter, providing strong and constitutive expression across a wide range of dividing and non-dividing cell types. The vector also includes a puromycin resistance gene (Puro) linked via an internal ribosome entry site (IRES), allowing efficient antibiotic selection and the generation of stable cell lines following transduction.

Packaged using a third-generation lentiviral system, LV-EF1α-CymR-IRES-Puro integrates efficiently into the host genome, enabling long-term and heritable expression of CymR. This vector is particularly well suited for establishing CymR-expressing “repressor” cell lines, which can be paired with CuO-regulated constructs to achieve tunable and inducible gene expression.

Supplied in a ready-to-use format, LV-EF1α-CymR-IRES-Puro is ideal for both in vitro and in vivo applications, including inducible gene regulation studies, synthetic biology workflows, functional genomics, and the development of stable mammalian cell models requiring precise temporal control of transgene expression.

Titer: >1E+9 TU/mL

Selection Antibiotic: Puromycin

Purity*: In vivo grade and OK for both in vitro tissue culture infection and in vivo injection

Size: 2 x 25 µL (Std Pack)

Storage Buffer: PBS

Infection Protocol:

Transfer Vector Map:

* Concentrated via PEG precipitation followed by ultra-centrifugation at 25000 rpm