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LV-CMV-SpCas9
Product Category: Pre-made recombinant lentivirus; LV-CMV-SpCas9; LV-CMV-Cas9; CMV-Cas9 Lentivirus
Description: LV-CMV-SpCas9 is a pre-made lentivirus that overexpresses SpCas9 under the CMV promoter and can be used along with guided RNA for genome editing. The Type II prokaryotic CRISPR/Cas system is the new class of tools for targeted genome engineering. Cas9 (CRISPR-associated protein 9) is an RNA-guided DNA nuclease enzyme associated with Streptococcus pyogenes’ CRISPR immunity system. The Cas9 nucleases derived from clustered regularly interspaced short palindromic repeats (CRISPR)-Cas systems use small RNAs as guides (gRNA) to cleave DNA in a sequence-specific manner. With its ease in designing guide sequences to target specific genomic loci, the CRISPR/Cas system is a much simpler, faster, and robust alternative to TALEN and Zinc finger nuclease platforms. Ready to use format.
Titer: >1E+9 TU/mL
Selection Antibiotic: None
Purity*: In vivo grade and OK for both in vitro tissue culture infection and in vivo injection
Size: 2 x 25 µL (Std Pack)
Storage Buffer: PBS
Infection Protocol: 
Transfer Vector Map:
* Concentrated via PEG precipitation followed by ultra-centrifugation at 25000 rpm
CITATIONS
| Shipping Weight | 6 lbs |
|---|---|
| Unit Size | 2 x 25 µL at >10⁹ TU/mL, 25 µL at >10⁹ TU/mL |
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