LV-CMV-mito-roGFP2-Orp1-PGK-BSD

Product Category: Pre-made recombinant lentivirus | LV-CMV-mito-roGFP2-Orp1-PGK-BSD | LV-CMV-mito-roGFP2-Orp1 | CMV-mito-roGFP2-Orp1 Lentivirus

Description:
LV-CMV-mito-roGFP2-Orp1-PGK-BSD is a pre-made, high-titer lentiviral vector designed to express a genetically encoded, mitochondria-targeted redox biosensor under the control of the strong CMV promoter. The construct also includes a blasticidin resistance gene driven by the PGK promoter, enabling efficient selection of stably transduced cells.

This vector encodes the mito-roGFP2-Orp1 sensor, which consists of a redox-sensitive GFP variant (roGFP2) fused to the yeast peroxidase Orp1 and targeted to mitochondria via a mitochondrial localization signal. Upon exposure to hydrogen peroxide (H₂O₂), Orp1 becomes oxidized and transfers the redox state to roGFP2, resulting in a shift in fluorescence excitation ratio. This enables sensitive, reversible, and ratiometric monitoring of mitochondrial H₂O₂ dynamics in real time.

Packaged in a replication-incompetent lentiviral system, LV-CMV-mito-roGFP2-Orp1 supports efficient transduction and stable genomic integration in both dividing and non-dividing cells. It is well-suited for long-term studies of mitochondrial oxidative stress, redox signaling, and cellular metabolism at the subcellular level.

Titer: >1E+9 TU/mL

Selection Antibiotic: Blasticidin

Purity*: In vivo grade and OK for both in vitro tissue culture infection and in vivo injection

Size: 2 x 25 µL (Std Pack)

Storage Buffer: PBS

Infection Protocol:

Transfer Vector Map:

* Concentrated via PEG precipitation followed by ultra-centrifugation at 25000 rpm