LV-CAG-GCaMP6f

Product Category: Pre-made recombinant lentivirus; LV-CAG-GCaMP6f; LV-CBA-GCaMP6f; CAG-GCaMP6f Lentivirus

Description:
GCaMP is a genetically encoded calcium indicator (GECI) and was created from a fusion of green fluorescent protein (GFP), calmodulin (CaM), and M13, a peptide sequence from myosin light chain kinase. When GECI binds Ca2+, the conformational change of GCaMP induces proximity of the GFP parts, eliciting a strong GFP fluorescence signal, which allows measuring action potentials and other receptor activation events that trigger Ca2+ influx. The advantage of GECI is that it can be genetically specified for studies in living organisms.

GCaMP6 is a novel ultra-sensitive format of GCaMP that outperformed other sensors in terms of accuracy and sensitivity in measuring cytosol Ca2+ levels. Mutations in calmodulin part of GCaMP6 created several variants which showed different calcium fluorescence decay rate (fast – GCaMP6f, slow – GCaMP6s, and medium – GCaMP6m).

LV-CAG-GCaMP6f is a pre-made lentivirus that expresses GCaMP6f under the CAG (also known as CBA) promoter. Ready to use format.

Titer: >1E+9 TU/mL

Selection Antibiotic: None

Purity*: In vivo grade and OK for both in vitro tissue culture infection and in vivo injection

Size: 2 x 25 µL (Std Pack)

Storage Buffer: PBS

Infection Protocol:

Transfer Vector Map:

* Concentrated via PEG precipitation followed by ultra-centrifugation at 25000 rpm