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GenMute™ siRNA Transfection Reagent for SMC
Description:
GenMute™ siRNA Transfection Reagent for Smooth Muscle Cells (SMC) is specifically pre-optimized for efficient delivery of siRNA into primary and cultured smooth muscle cells. Smooth muscle cells are found in the walls of blood vessels and hollow organs and play essential roles in regulating vascular tone, blood pressure, and organ contractility. However, SMCs are often difficult to transfect using conventional methods due to their low proliferation rate and sensitivity to cytotoxic reagents. This reagent enables high siRNA uptake with minimal toxicity, making it well-suited for gene silencing studies involving vascular biology, cardiovascular disease, airway function, and smooth muscle physiology in vitro.
Refer to the following optimal transfection conditions for maximal silencing on the SMC. GenMute™ reagent, 1.0 mL, is sufficient for ~833 transfections in 24-well plates or ~416 transfections in 6-well plates.
| Summary of Optimal Transfection Conditions: | |
| Cell culture medium | DMEM with 4.5 g/L glucose, 10% FBS |
| Confluency of cell on the day of transfection | ~60% |
| Optimal siRNA concentration | 40 nM |
| Diluent for siRNA and GenMute™ Reagent | 1 x GenMute™ Transfection Buffer |
| Incubation Time to Form GenMute™/siRNA Complex | ~15 min at RT |
| Maximal Silencing Timeline | 72 hrs |
| Reporter Gene Silencing Efficiency (% ) | Renilla Luciferase 85% |
Storage: Store at 4 °C. If stored properly, the product is stable for 24 months or longer
Transfection Protocol 
CITATIONS
| Shipping Weight | 6 lbs |
|---|---|
| Unit Size | 5 x 1.0 mL, 1.0 mL, 0.5 mL, 0.1 mL |
Partnership Opportunities
We offer OEM manufacturing services for our transfection reagents to qualified life science reagent companies for resale under their own brand names. Interested partners are encouraged to contact us for additional information regarding OEM collaboration opportunities.
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