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GenMute™ siRNA Transfection Reagent for SH-SY5Y Cell
Description:
GenMute™ siRNA Transfection Reagent for SH-SY5Y Cells is specifically optimized for efficient siRNA delivery into SH-SY5Y human neuroblastoma cells. SH-SY5Y cells are widely used as an in vitro neuronal model because they can be maintained in an undifferentiated state or induced to differentiate into neuron-like cells using agents such as retinoic acid or brain-derived neurotrophic factor (BDNF). These cells are commonly employed in studies of neuronal development, synaptic function, neurodegenerative diseases, neurotoxicity, and drug discovery. The optimized formulation enables robust gene knockdown while maintaining high cell viability in both proliferating and differentiated SH-SY5Y cultures.
Size & content:
– GenMute™ Reagent, 1.0 mL, sufficient for ~833 reactions based on transfecting 25 pmol siRNA or miRNA mimics in a 24-well plate
– GenMute™ Transfection Buffer (5x ), formulated for maximal transfection efficiency, 8.0 mL to make 40 mL working solution
Refer to the following optimal transfection conditions for maximal silencing on primary macrophages. GenMute™ reagent, 1.0 mL, is sufficient for ~833 transfections in 24-well plates or ~416 transfections in 6-well plates.
| Summary of Optimal Transfection Conditions: | |
| Cell culture medium | DMEM with 4.5 g/L glucose, 10% FBS |
| Confluency of cell on the day of transfection | ~70% |
| Optimal siRNA concentration | 40 nM |
| Diluent for siRNA and GenMute™ Reagent | 1 x GenMute™ Transfection Buffer |
| Incubation Time to Form GenMute™/siRNA Complex | ~15 min at RT |
| Maximal Silencing Timeline | 72 hrs |
| Reporter Gene Silencing Efficiency (% ) | 86% |
Storage: Store at 4 °C. If stored properly, the product is stable for 24 months or longer
Transfection Protocol 
CITATIONS
| Shipping Weight | 6 lbs |
|---|---|
| Unit Size | 5 x 1.0 mL, 1.0 mL, 0.5 mL, 0.1 mL |
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Partnership Opportunities
We offer OEM manufacturing services for our transfection reagents to qualified life science reagent companies for resale under their own brand names. Interested partners are encouraged to contact us for additional information regarding OEM collaboration opportunities.
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