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GenMute™ siRNA Transfection Reagent for Primary Neurons
Description:
GenMute™ siRNA Transfection Reagent for Primary Neurons is specifically optimized for efficient siRNA delivery into primary neuronal cultures, which are typically difficult to transfect. Using pre-validated transfection conditions, the GenMute™ reagent has been shown to achieve up to 80% gene silencing in primary neurons at a final siRNA concentration of 50 nM, while maintaining high cell viability.
Size & content:
– GenMute™ Reagent, 1.0 mL, sufficient for ~833 reactions based on transfecting 25 pmol siRNA or miRNA mimics in a 24-well plate
– GenMute™ Transfection Buffer (5x ), formulated for maximal transfection efficiency, 8.0 mL to make 40 mL working solution
Refer to the following optimal transfection conditions for maximal silencing on primary neurons. GenMute™ reagent, 1.0 mL, is sufficient for ~833 transfections in 24-well plates or ~416 transfections in 6-well plates.
| Summary of Optimal Transfection Conditions: | |
| Cell Culture Medium | Neurobasal plus B27 serum-free medium |
| Cell Confluency on the day of transfection | ~90% |
| Optimal siRNA Concentration | 30 nM |
| Diluent for siRNA and GenMute™ Reagent | 1 x GenMute™ Transfection Buffer |
| Incubation Time to Form GenMute™/siRNA Complex | ~15 min at RT |
| Maximal Silencing Timeline | ~72 hrs |
| Gene Silencing Efficiency (% ) | 80% |
Storage: Store at 4 °C. If stored properly, the product is stable for 24 months or longer
Transfection Protocol 
CITATIONS
| Shipping Weight | 6 lbs |
|---|---|
| Unit Size | 5 x 1.0 mL, 1.0 mL, 0.5 mL, 0.1 mL |
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Partnership Opportunities
We offer OEM manufacturing services for our transfection reagents to qualified life science reagent companies for resale under their own brand names. Interested partners are encouraged to contact us for additional information regarding OEM collaboration opportunities.
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