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GenMute™ siRNA Transfection Reagent for Primary Macrophages
Description:
GenMute™ siRNA Transfection Reagent for PMG is specifically optimized for efficient siRNA delivery into primary macrophages. Macrophages are tissue-resident white blood cells derived from circulating monocytes and play central roles in both innate and adaptive immunity. As professional phagocytes, they engulf and degrade cellular debris, pathogens, and foreign particles, and secrete cytokines that regulate immune responses. Primary macrophages can be identified by the expression of characteristic markers, including CD14, CD11b, F4/80 (mouse) or EMR1 (human), Lysozyme M, MAC-1/MAC-3, and CD68, detectable by flow cytometry or immunohistochemical staining.
Size & content:
– GenMute™ Reagent, 1.0 mL, sufficient for ~833 reactions based on transfecting 25 pmol siRNA or miRNA mimics in a 24-well plate
– GenMute™ Transfection Buffer (5x ), formulated for maximal transfection efficiency, 8.0 mL to make 40 mL working solution
Refer to the following optimal transfection conditions for maximal silencing on primary macrophages. GenMute™ reagent, 1.0 mL, is sufficient for ~833 transfections in 24-well plates or ~416 transfections in 6-well plates.
| Summary of Optimal Transfection Conditions: | |
| Cell culture medium | DMEM with 4.5 g/L glucose, 10% FBS |
| Confluency of cell on the day of transfection | ~80% |
| Optimal siRNA concentration | 40 nM |
| Diluent for siRNA and GenMute™ Reagent | 1 x GenMute™ Transfection Buffer |
| Incubation Time to Form GenMute™/siRNA Complex | ~15 min at RT |
| Maximal Silencing Timeline | 72 hrs |
| Reporter Gene Silencing Efficiency (% ) | 85% |
Storage: Store at 4 °C. If stored properly, the product is stable for 24 months or longer
Transfection Protocol 
CITATIONS
| Shipping Weight | 6 lbs |
|---|---|
| Unit Size | 5 x 1.0 mL, 1.0 mL, 0.5 mL, 0.1 mL |
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Partnership Opportunities
We offer OEM manufacturing services for our transfection reagents to qualified life science reagent companies for resale under their own brand names. Interested partners are encouraged to contact us for additional information regarding OEM collaboration opportunities.
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