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GenMute™ siRNA Transfection Reagent for Primary Keratinocytes
Description:
GenMute™ siRNA Transfection Reagent for Primary Keratinocytes is specifically pre-optimized for efficient delivery of siRNA into primary keratinocytes. These cells are well known for their resistance to conventional DNA and siRNA transfection methods due to their limited proliferation and sensitivity to cytotoxic reagents. Using the recommended pre-optimized conditions, this formulation can achieve robust gene knockdown, with average silencing efficiencies of up to ~90% while maintaining high cell viability. This makes the reagent well-suited for gene function studies, skin biology research, and dermatological applications in primary keratinocyte cultures.
Size & content:
– GenMute™ Reagent, 1.0 mL, sufficient for ~833 reactions based on transfecting 20 pmol siRNA or miRNA mimics in a 24-well plate
– GenMute™ Transfection Buffer (5x ), formulated for maximal transfection efficiency, 8.0 mL to make 40 mL working solution
Refer to the following optimal transfection conditions for maximal silencing on primary keratinocytes. GenMute™ reagent, 1.0 mL, is sufficient for ~833 transfections in 24-well plates or ~416 transfections in 6-well plates.
| Summary of OptimalTransfectionConditions: | |
| Cell Culture Medium | DMEM with 4.5 g/L glucose, 10% FBS |
| Cell confluency on the day of transfection | ~70% |
| Optimal siRNA Concentration | 40 nM |
| Diluent for siRNA and GenMute™ Reagent | 1 x GenMute™ Transfection Buffer |
| Incubation Time to Form GenMute™/siRNA Complex | ~15 min at RT |
| Maximal Silencing Timeline | 72 hrs |
| Reporter Gene Silencing Efficiency (% ) | Renilla Luciferase 90% |
Storage: Store at 4 °C. If stored properly, the product is stable for 24 months or longer
Transfection Protocol 
CITATIONS
| Shipping Weight | 6 lbs |
|---|---|
| Unit Size | 5 x 1.0 mL, 1.0 mL, 0.5 mL, 0.1 mL |
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Partnership Opportunities
We offer OEM manufacturing services for our transfection reagents to qualified life science reagent companies for resale under their own brand names. Interested partners are encouraged to contact us for additional information regarding OEM collaboration opportunities.
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