-
PHONE: 1-866-918-6812
GenMute™ siRNA Transfection Reagent for Jurkat Cell
Description:
GenMute™ siRNA Transfection Reagent for Jurkat Cells is pre-optimized for efficient delivery of siRNA into Jurkat cells, an immortalized human T-lymphocyte cell line widely used in immunology and cancer research. Jurkat cells serve as a key model for studying acute T-cell leukemia, T-cell receptor signaling, apoptosis, and the expression of chemokine receptors involved in viral entry, including HIV. They are also commonly used to investigate interleukin-2 (IL-2) production and immune activation pathways. This reagent enables high transfection efficiency with minimal cytotoxicity in these suspension cells, making it well suited for gene silencing studies, pathway analysis, and functional assays in vitro.
Size & content:
– GenMute™ Reagent, 1.0 mL, sufficient for ~833 reactions based on transfecting 10 pmol siRNA or miRNA mimics in a 24-well plate
– GenMute™ Transfection Buffer (5x ), formulated for maximal transfection efficiency, 8.0 mL to make a 40 mL working solution
Refer to the following optimal transfection conditions for maximal silencing on Jurkat cells. GenMute™ reagent, 1.0 mL, is sufficient for ~833 transfections in 24-well plates or ~416 transfections in 6-well plates.
| Summary of Optimal Transfection Conditions: | |
| Cell Culture Medium | 1640 Medium with 10% FBS |
| Cell confluency on the day of transfection | ~70% |
| Optimal siRNA Concentration | 40 nM |
| Diluent for siRNA and GenMute™ Reagent | 1 x GenMute™ Transfection Buffer |
| Incubation Time to Form GenMute™/siRNA Complex | ~15 min at RT |
| Maximal Silencing Timeline | 72 hrs |
| Reporter Gene Silencing Efficiency (% ) | Renilla Luciferase 80% |
Storage: Store at 4 °C. If stored properly, the product is stable for 24 months or longer
Transfection Protocol 
CITATIONS
| Shipping Weight | 6 lbs |
|---|---|
| Unit Size | 5 x 1.0 mL, 1.0 mL, 0.5 mL, 0.1 mL |
Customers who bought this product also purchased…
-
SL100816AAV-CAG-GFP (AAV Serotype 2)$488.00Add to cart This product has multiple variants. The options may be chosen on the product page
-
SL100499GenJet™ Plus DNA In Vitro Transfection Reagent Original price was: $218.00.$185.00Current price is: $185.00.Add to cart This product has multiple variants. The options may be chosen on the product page
-
SL100817AAV-Synapsin-GFP (AAV Serotype 2)$488.00Add to cart This product has multiple variants. The options may be chosen on the product page
-
SL100706Ad-Cre-GFP (co-expression of Cre recombinase and GFP)$488.00Add to cart This product has multiple variants. The options may be chosen on the product page
-
SL100268LV-CMV-GFP-Puro$468.00Add to cart This product has multiple variants. The options may be chosen on the product page
-
SL100568-PMGGenMute™ siRNA Transfection Reagent for Primary Macrophages$218.00Add to cart This product has multiple variants. The options may be chosen on the product page
-
SL100568-PNNGenMute™ siRNA Transfection Reagent for Primary Neurons$218.00Add to cart This product has multiple variants. The options may be chosen on the product page
Partnership Opportunities
We offer OEM manufacturing services for our transfection reagents to qualified life science reagent companies for resale under their own brand names. Interested partners are encouraged to contact us for additional information regarding OEM collaboration opportunities.
Important updates waiting for you!
Enter your email address below and subscribe to our newsletter