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GenMute™ siRNA Transfection Reagent for HepG2 Cell
Description:
GenMute™ siRNA Transfection Reagent for HepG2 Cells is pre-optimized for efficient delivery of siRNA into HepG2 cells, a human hepatocellular carcinoma cell line widely used in liver biology, metabolism, toxicology, and drug discovery studies. HepG2 cells were originally derived from the liver tissue of a 15-year-old Caucasian male with well-differentiated hepatocellular carcinoma. These epithelial-like cells are non-tumorigenic in nude mice and retain many liver-specific functions, including the secretion of major plasma proteins such as albumin, α2-macroglobulin, α1-antitrypsin, transferrin, and plasminogen. This reagent enables high transfection efficiency with low cytotoxicity, making it well-suited for gene silencing and functional assays in vitro.
Size & content:
– GenMute™ Reagent, 1.0 mL, sufficient for ~833 reactions based on transfecting 25 pmol siRNA or miRNA mimics in a 24-well plate
– GenMute™ Transfection Buffer (5x ), formulated for maximal transfection efficiency, 8.0 mL to make 40 mL working solution
Refer to the following optimal transfection conditions for maximal silencing on HepG2 cells. GenMute™ reagent, 1.0 mL, is sufficient for ~833 transfections in 24-well plates or ~416 transfections in 6-well plates.
| Summary of Optimal Transfection Conditions: | |
| Cell culture medium | DMEM with 4.5 g/L glucose, 10% FBS |
| Cell confluency on the day of transfection | ~50% |
| Optimal siRNA concentration | 40 nM |
| Diluent for siRNA and GenMute™ Reagent | 1 x GenMute™ Transfection Buffer |
| Incubation Time to Form GenMute™/siRNA Complex | ~15 mins at RT |
| Maximal Silencing Timeline | 72 hrs |
| Reporter Gene Silencing Efficiency (% ) | Renilla Luciferase 85% |
Storage: Store at 4 °C. If stored properly, the product is stable for 24 months or longer
Transfection Protocol 
CITATIONS
| Shipping Weight | 6 lbs |
|---|---|
| Unit Size | 5 x 1.0 mL, 1.0 mL, 0.5 mL, 0.1 mL |
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Partnership Opportunities
We offer OEM manufacturing services for our transfection reagents to qualified life science reagent companies for resale under their own brand names. Interested partners are encouraged to contact us for additional information regarding OEM collaboration opportunities.
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