GenMute™ siRNA Transfection Reagent for HeLa Cell

Description:
GenMute™ siRNA Transfection Reagent for HeLa Cells is specifically pre-optimized for efficient delivery of siRNA into HeLa cells, one of the most widely used human cervical cancer cell lines in biomedical research. HeLa cells are extensively employed in studies of gene function, cancer biology, signal transduction, virology, and drug discovery, but achieving consistent knockdown with minimal toxicity can be challenging with general-purpose reagents. This formulation is engineered to maximize cellular uptake and intracellular release of siRNA while preserving cell viability and normal morphology. It delivers high transfection efficiency with low cytotoxicity, enabling robust and reproducible gene silencing for downstream applications such as pathway analysis, target validation, and functional assays in vitro.

Size & content:
– GenMute™ Reagent, 1.0 mL, sufficient for ~833 reactions based on transfecting 20 pmol siRNA or miRNA mimics in a 24-well plate
– GenMute™ Transfection Buffer (5x ), formulated for maximal transfection efficiency, 8.0 mL to make 40 mL working solution

Refer to the following optimal transfection conditions for maximal silencing on HeLa cells. GenMute™ reagent, 1.0 mL, is sufficient for ~833 transfections in 24-well plates or ~416 transfections in 6-well plates.

Storage: Store at 4 °C. If stored properly, the product is stable for 24 months or longer

Transfection Protocol