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GenMute™ siRNA Transfection Reagent for C2C12 Cell
Description:
GenMute™ siRNA Transfection Reagent for C2C12 Cells is pre-optimized for efficient siRNA delivery into C2C12 myoblasts. The C2C12 cell line was originally established by Yaffe and Saxel (1977) through selective serial passaging of myoblasts isolated from the thigh muscle of C3H mice following crush injury. These cells retain the ability to proliferate as myoblasts and to differentiate into multinucleated myotubes under appropriate conditions. C2C12 cells are widely used as an in vitro model for skeletal muscle development, myogenesis, muscle metabolism, and signaling pathways, including studies of myosin regulation and androgen receptor (AR) expression.
Size & content:
– GenMute™ Reagent, 1.0 ml, sufficient for ~833 reactions based on transfecting 20 pmol siRNA or miRNA mimics in a 24-well plate
– GenMute™ Transfection Buffer (5x ), formulated for maximal transfection efficiency, 8.0 mL to make 40 mL working solution
Refer to the following optimal transfection conditions for maximal silencing on C2C12 cells. GenMute™ reagent, 1.0 ml, is sufficient for ~833 transfections in 24-well plates or ~416 transfections in 6-well plates.
| Summary of Optimal Transfection Conditions: | |
| Cell culture medium | DMEM with 4.5 g/L glucose, 10% FBS |
| Confluency of the cell on the day of transfection | ~50% |
| Optimal siRNA concentration | 40 nM |
| Diluent for siRNA and GenMute™ Reagent | 1 x GenMute™ Transfection Buffer |
| Incubation Time to Form GenMute™/siRNA Complex | ~15 min at RT |
| Maximal Silencing Timeline | 72 hrs |
| Reporter Gene Silencing Efficiency (% ) | Renilla Luciferase 80% |
Storage: Store at 4 °C. If stored properly, the product is stable for 24 months or longer
Transfection Protocol 
CITATIONS
| Shipping Weight | 6 lbs |
|---|---|
| Unit Size | 5 x 1.0 mL, 1.0 mL, 0.5 mL, 0.1 mL |
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Partnership Opportunities
We offer OEM manufacturing services for our transfection reagents to qualified life science reagent companies for resale under their own brand names. Interested partners are encouraged to contact us for additional information regarding OEM collaboration opportunities.
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