GenJet™ In Vitro DNA Transfection Reagent

Description:
GenJet™ DNA In Vitro Transfection Reagent is a powerful and reliable transfection reagent designed to deliver high transfection efficiency with minimal, often undetectable, cytotoxicity. GenJet™ is formulated using a unique chemistry that covalently cross-links cationic lipids with polymers, resulting in exceptional performance and clearly distinguishable advantages over conventional transfection reagents.

GenJet™ has been demonstrated to efficiently deliver genes into a wide range of established cell lines as well as primary cells, including HEK293, 293T, 293E, CHO, COS-1, HeLa, NIH/3T3, insect cell lines (Sf9 and Sf21), and many other eukaryotic cell types.

One 1.0 mL vial of GenJet™ Reagent is sufficient for approximately 666 transfections in 24-well plates or 333 transfections in 6-well plates.

Applications:
– Efficient delivery of DNA into mammalian and insect cells
– Dramatically enhances viral infection (up to 200-fold), including replication-deficient adenovirus, in hard-to-transfect cells

Features:
– Enables exceptionally high viral titers
– Highly effective for transfection of large DNA constructs (up to 56 kb)
– Performs equally well for single or co-transfection of multiple DNA constructs
– Supports exceptionally high levels of recombinant protein expression
– Ideal for hard-to-transfect cell types
– Cost-effective transfection solution

Storage Conditions:
Store at 4 °C. When stored under recommended conditions, the product is stable for 12 months or longer.

Broad Transfection Spectrum

Comparisons of Transfection Efficiency and Cytotoxicity of GenJet™ DNA In Vitro Transfection Reagent with the Leading Brand Name Products
Exceptional transfection efficiency of extremely long DNA by GenJet™ DNA In Vitro Transfection Reagent. HEK293 cells were transfected with GFP-tagged DNA (total 14 kb) by GenJet™ (upper panel) and FuGene™ 6 (lower panel), respectively. The cells were visualized by Nikon Eclipse Fluorescence microscope with DIC phase (left panel) and FITC (right panel) imaging 24 hours post-transfection

Exceptionally high titers of lentivirus were generated from 293F cells. Three DNAs co-transfected with GenJet™ In Vitro DNA Transfection Reagent (left panel) in comparison of LipoFectamine 2000™ (L2K, right panel). 100 microliter virus supernatant from 293F cells was used to counter-infect 293F cells, which were then passed through FACS. The numbers in the upper right corner indicate the percentage of transduced cells. The titers of lentivirus generated with GenJet™ and L2K were quantified to be 7.9×10⁶ and 3.1×10⁶ tu/mL, respectively

Primary Murine Skin Fibroblast transfected with pEGFP-C1 plasmid using GenJet™ In Vitro DNA Transfection Reagent (upper panel) and the most popular brand product FuGene™ HD (lower panel). The cells were visualized by a Nikon Eclipse Fluorescence microscope with DIC phase imaging (left panel) and FITC imaging (right panel) 24 hours post-transfection

Primary Murine Keratinocytes transfected with pEGFP-C1 plasmid using GenJet™ In Vitro DNA Transfection Reagent (upper panel) and one of the most popular brand products, FuGene™ HD (lower panel). The cells were visualized by Nikon Eclipse Fluorescence microscope with DIC phase imaging (left panel) and FITC imaging (right panel) 24 hours post-transfection

Comparison of cytotoxicity of GenJet™ DNA In Vitro Transfection Reagent with L2K™ on primary murine skin fibroblast. The primary murine fibroblast was incubated with the indicated transfection reagents/pEGFP-C1 (DNA) complexes above for 4 hours in serum-free DMEM High Glucose medium, followed by replacement of complete serum-containing medium. The cells were visualized by Nikon Eclipse Fluorescence microscope with DIC phase imaging 24 hours post-transfection

Technical Information & Transfection Protocols
– A General Protocol for Mammalian Cell Transfection
– A Short Protocol for Advanced Users
– A Protocol for Lentivirus Packaging in HEK293T Cells 
– A Protocol for AAV Packaging in HEK293T Cells 
– A Protocol for Adenovirus Packaging in HEK293A Cells
– A Protocol for Trasnfecting Bacmids to Sf9 Cells