GenJet™ In Vitro DNA Transfection Reagent (Ver. II)

Description:
GenJet™ In Vitro DNA Transfection Reagent (Ver. II) is an upgraded formulation of the original GenJet™ In Vitro DNA Transfection Reagent. Using a new chemistry, GenJet™ (Ver. II) liberates a higher proportion of DNA-condensing groups (100% compared to 80% in the previous version), resulting in a 3–4 fold increase in transfection efficiency without compromising cell viability.

One 1.0 mL vial of GenJet™ (Ver. II) Reagent is sufficient for approximately 667 transfections in 24-well plates or 333 transfections in 6-well plates.

Features Compared with the Original GenJet™ In Vitro DNA Transfection Reagent
– Cell type–dependent 3–4 fold higher transfection efficiency
– Preferred choice for hard-to-transfect cells
– Enhanced transfection efficiency in the presence of serum and antibiotics for most cell types
– Enables exceptionally high levels of recombinant protein expression

Storage Conditions:
Store at 4 °C. When stored under recommended conditions, the product remains stable for 12 months or longer.

Performance Comparison:
Comparison of transfection efficiency and cytotoxicity of GenJet™ (Ver. II) with the original GenJet™, as well as other leading transfection reagents.

Comparison of transfection efficiency (left panel) and toxicity (right panel) GenJet™ Ver. II with the old version of GenJet™ on commonly used mammalian cells. phRL-CMV encoding Renilla Luciferase was introduced into cells in the presence of serum and antibiotics. The Renilla Luciferase activity was determined with the Renilla Luciferase Assay System (Promega). For cytotoxicity, the MTT assay was used to determine cell viability, with the untransfected control set at 100%.

A comparison of the transfection efficiency of GenJet™ (Ver. II) vs. Lipofectamine 2000 on primary rat aortic smooth muscle cells. Primary rat aortic smooth muscle cells were prepared and transfected with a nuclear-targeted GFP cDNA by GenJet™ (right panel) and Lipofectamine 2000 (L2K, left panel), respectively. The transfection efficiency was checked under a Nikon Eclipse 2000 fluorescence microscope 48 hours post-transfection

Upper Panel: Transfection efficiency comparison of GenJet™ Ver. II with the old version of GenJet™ on 293T cells. pEGFP-N3 was transfected into 293T cells at 90% confluency in the presence of serum and antibiotics. GFP fluorescence was detected with Nikon Eclipse 2000 microscopy 48 hours post-transfection
Lower panel: Transfection efficiency comparison of GenJet™ Ver. II with the old version of GenJet™ on Hela cells. pEGFP-N3 was transfected into Hela cells at 90% in the presence of serum and antibiotics. GFP fluorescence was detected with Nikon Eclipse 2000 microscopy 48 hours post-transfection

Technical Information & Transfection Protocols
– A General Protocol for Transfecting Mammalian Cells
– A Short Protocol for Advanced Users
– A Protocol for Lentivirus Packaging in HEK293T Cells
– A Protocol for rAAV Packaging in HEK293T Cells
– A Protocol for Adenovirus in HEK293A Cells
– A Protocol for Transfecting Suspension 293 and CHO Cells
– Technical Note & Transfection Tips