CalFectin™ Mammalian Cell Transfection Reagent

Description:
CalFectin™ Mammalian DNA Transfection Reagent is a pre-optimized and refined calcium phosphate–based transfection reagent featuring a novel chemistry. Compared to traditional calcium phosphate methods, CalFectin™ contains a significantly lower concentration of calcium, resulting in markedly reduced cytotoxicity without compromising transfection efficiency.

CalFectin™ Reagent is highly effective for both transient and long-term stable transfection in most adherent mammalian cell lines. Compared with liposome- and polymer-based transfection reagents, CalFectin™ demonstrates superior performance and higher efficiency in a wide range of cell types, including HepG2, HEK293, CHO, HeLa, COS, NIH/3T3, LNCaP, C6, PC12, and primary cultured cells.

One 1.0 mL vial of CalFectin™ Reagent is sufficient for approximately 666 transfections in 24-well plates or 333 transfections in 6-well plates.

Application:
High-efficiency stable or transient transfection of eukaryotic cells.

Feature:
– High efficient: Components optimized for high transfection efficiencies
– Simple procedures: Total one tube reaction

Storage Condition
Store at 4 °C. If stored properly, the product is stable for 12 months or longer.

Comparisons of Transfection Efficiency of CalFectin™ Reagent with the Leading Brand Name Product
A comparison study showing transfection efficiency of CalFectin™ Reagent vs. a leading product, Lipofectamine 2000 on HEK293T cells. HEK293T cells were transfected with GFP-tagged DNA (total 14 kb) by CalFectin™ (left panel) and Lipofectamine 2000 (right panel), respectively. The cells were visualized by a Nikon Eclipse Fluorescence microscope 24 hours post-transfection

A comparison study showing the transfection efficiency of CalFectin™ Reagent vs. a leading product, Lipofectamine 2000 on HepG2 cells. HepG2 cells were transfected with GFP vector (pEGFP-N3) by CalFectin™ (left panel) and Lipofectamine 2000 (right panel), respectively. The cells were visualized by a Nikon Eclipse Fluorescence microscope 24 hours post-transfection

Technical Information & Datasheet
– A General Protocol for Transfecting Mammalian Cells
– A Short Protocol for Advanced Users
– A Protocol for Lentivirus Packaging in HEK293T Cells
– A Protocol for AAV Packaging in HEK293T Cells
– A Protocol for Adenovirus Packaging in HEK293A Cells