BlotFresh™ Western Blot Stripping Reagent (Ver. II)

Description:
BlotFresh™ Western Blot Stripping Reagent (Ver. II) is a ready-to-use, mild yet highly effective solution for removing primary and secondary antibodies from Western blot membranes without damaging immobilized proteins. This optimized formulation enables multiple rounds of re-probing on the same PVDF or nitrocellulose membrane, allowing detection of additional targets or loading controls while conserving valuable samples.

BlotFresh™ (Ver. II) efficiently strips antibodies in minutes under gentle conditions, preserving antigen integrity and minimizing loss of low-abundance proteins. The reagent is compatible with chemiluminescent and fluorescent detection methods and does not require heating or harsh chemicals, reducing background and membrane degradation.

Ideal for routine Western blot workflows, BlotFresh™ (Ver. II) improves experimental efficiency, reproducibility, and cost-effectiveness by enabling reliable membrane reuse across multiple probing cycles.

Features:
– Strip blot in 5~15 minutes
– Incubation at room temperature
– No stench smelling without addition of 2-mercaptoethanol or its analogs
– A propriety formulation with an eco-friendly and non-hazardous organic solvent (with light odor) included to completely disassociate the bound antibodies

Data Sheet & Protocol