Ad-GCaMP6f

Product Category: Human Adenovirus Type5 (dE1/E3); Ad-CMV-GCaMP6f; Ad-GCaMP6f
Description:
GCaMP is a genetically encoded calcium indicator (GECI) and created from a fusion of green fluorescent protein (GFP), calmodulin (CaM), and M13, a peptide sequence from myosin light chain kinase. When GECI binds Ca2+, the conformational change of GCaMP induces proximity of the GFP parts, eliciting strong GFP fluorescence signal, which allows measuring action potentials and other receptor activation events that trigger Ca2+ influx. The advantage of GECI's are that they can be genetically specified for studies in living organisms.
GCaMP6 is a novel ultra-sensitive format of GCaMP that outperformed other sensors in terms of accuracy and sensitivity in measuring cytosol Ca2+ level. Mutations in calmodulin part of GCaMP6 created several variants which showed different calcium fluorescence decay rate (fast – GCamP6f, slow – GCaMP6s and medium – GCaMP6m).
This recombinant human adenovirus type 5 expresses genetically encoded calcium indicator GCaMP6f under CMV promoter.
Titer: 1E+10 ~ 1E+11 PFU/mL
Storage Buffer: DMEM with 2% BSA & 2.5% Glycerol
CITATIONS
| Shipping Weight | 6 lbs |
|---|---|
| Unit Size | 200 µL at >1E+10 PFU/mL, 50 µL at >1E+10 PFU/mL |
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