SignaGen Ad-GCaMP6f premade adenoviral vector map
Ad-GCaMP6f

Price range: $156.00 through $488.00

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Ad-GCaMP6f

SKU: SL101142 Category:
  • Experimentally Validated
  • Reproducible Performance
  • Dedicated Scientific Support
  • Customization Options Available

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Customize promoter, transgene, reporter, or tag

Product Category: Human Adenovirus Type5 (dE1/E3); Ad-CMV-GCaMP6f; Ad-GCaMP6f

Description:
GCaMP is a genetically encoded calcium indicator (GECI) and created from a fusion of green fluorescent protein (GFP), calmodulin (CaM), and M13, a peptide sequence from myosin light chain kinase. When GECI binds Ca2+, the conformational change of GCaMP induces proximity of the GFP parts, eliciting strong GFP fluorescence signal, which allows measuring action potentials and other receptor activation events that trigger Ca2+ influx. The advantage of GECI's are that they can be genetically specified for studies in living organisms.

GCaMP6 is a novel ultra-sensitive format of GCaMP that outperformed other sensors in terms of accuracy and sensitivity in measuring cytosol Ca2+ level. Mutations in calmodulin part of GCaMP6 created several variants which showed different calcium fluorescence decay rate (fast – GCamP6f, slow – GCaMP6s and medium – GCaMP6m).

This recombinant human adenovirus type 5 expresses genetically encoded calcium indicator GCaMP6f under CMV promoter.

Titer: 1E+10 ~ 1E+11 PFU/mL

Storage Buffer: DMEM with 2% BSA & 2.5% Glycerol

User Manual:

Shuttle Vector Map:
SignaGen Ad-GCaMP6f premade adenoviral vector construct map showing GCaMP6f calcium indicator and adenoviral vector elements

CITATIONS

Shipping Weight6 lbs
Unit Size

200 µL at >1E+10 PFU/mL, 50 µL at >1E+10 PFU/mL

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