Ad-CMV-mCherry-GFP-LC3

Product Category: Human Adenovirus Type 5 (ΔE1/ΔE3) | Ad-CMV-mCherry-GFP-LC3
Description:
Ad-CMV-mCherry-GFP-LC3 is a pre-made recombinant adenoviral vector based on a serotype 5 backbone with E1 and E3 deletions. It drives expression of an mCherry–GFP–LC3 fusion protein under the control of the CMV promoter in mammalian cells. This dual-fluorescent reporter enables visualization and assessment of autophagic flux: autophagosomes exhibit both GFP and mCherry signals, while autolysosomes display mCherry fluorescence only due to the acid-sensitive quenching of GFP within lysosomes. This vector is widely used for monitoring autophagy dynamics in vitro and in vivo.
Titer: 1E+10 ~ 1E+11 PFU/mL
Storage Buffer: DMEM with 2% BSA & 2.5% Glycerol
Shuttle Vector Map:
CITATIONS
| Shipping Weight | 6 lbs |
|---|---|
| Unit Size | 200 µL at >1E+10 PFU/mL, 50 µL at >1E+10 PFU/mL |
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