Ad-CMV-Cas9(D10A)-T2A-DasherGFP

Product Category: Human Adenovirus Type 5 (ΔE1/E3) | Ad-CMV-Cas9(D10A)-T2A-GFP | Ad-CMV-Cas9(D10A)-T2A-DasherGFP

Description:
Ad-CMV-Cas9(D10A)-T2A-DasherGFP is a recombinant, E1/E3-deleted human adenovirus serotype 5 vector engineered to express a codon-optimized Streptococcus pyogenes Cas9(D10A) nickase under the control of the CMV immediate early promoter. The Cas9(D10A) variant contains a point mutation in the RuvC nuclease domain, converting the wild-type Cas9 into a DNA nickase that introduces single-strand breaks (nicks) instead of double-strand breaks.

This vector co-expresses DasherGFP via a self-cleaving T2A peptide, enabling real-time monitoring of transduction efficiency and transgene expression in mammalian cells. The Cas9 nickase can be used in combination with paired guide RNAs (gRNAs) to generate offset single-strand nicks on opposite DNA strands, resulting in high-specificity genome editing with significantly reduced off-target effects compared to wild-type Cas9.

The adenoviral delivery system enables efficient transduction across a broad range of dividing and non-dividing cell types, providing robust transient expression without genomic integration. This makes the vector particularly suitable for applications requiring precise genome modification, reduced off-target activity, or short-term Cas9 expression.

Key Features:

• Expresses Cas9(D10A) nickase for high-fidelity genome editing
• Co-expression of DasherGFP via T2A for easy visualization and tracking
• CMV promoter drives strong, ubiquitous expression in mammalian cells
• E1/E3-deleted Ad5 backbone for replication-deficient, high-efficiency delivery
• Supports paired gRNA strategies for enhanced targeting specificity
• Broad tropism for both dividing and non-dividing cells
• Non-integrating system enabling transient expression

Applications:

• High-specificity genome editing using paired gRNAs
• Reduction of off-target cleavage events
• Gene correction and precise genome engineering studies
• Functional genomics and pathway analysis
• Optimization of CRISPR nickase-based systems in vitro and in vivo

Titer: 1E+10 ~ 1E+11 PFU/mL

Storage Buffer: DMEM with 2% BSA & 2.5% Glycerol

User Manual:

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