AAV-Mecp2-SpCas9(D10A) (Serotype 1)

Product Category: Pre-made recombinant AAV | AAV1-Mecp2-SpCas9(D10A) | AAV1-Mecp2-Cas9(Nickase)

Description:
AAV1-Mecp2-SpCas9(D10A) is a pre-packaged, replication-deficient recombinant adeno-associated virus designed for neuron-targeted delivery of the Cas9 nickase variant in mammalian systems. This vector utilizes the AAV serotype 1 capsid for efficient transduction of neuronal tissues, while the Mecp2 promoter drives selective expression in neurons, enabling precise genetic manipulation in the central nervous system.

The construct encodes the SpCas9(D10A) nickase, a mutant form of Streptococcus pyogenes Cas9 in which one nuclease domain is inactivated, resulting in single-strand DNA cleavage. When paired with appropriately designed dual gRNAs, this system enables high-specificity genome editing with reduced off-target effects compared to wild-type Cas9, making it particularly suitable for sensitive in vivo applications.

Packaged using a high-quality AAV production system and purified to in vivo grade, AAV1-Mecp2-SpCas9(D10A) is ideal for neuroscience research, including gene function studies, neural circuit analysis, and precision genome engineering in neuronal populations. The product is provided in a ready-to-use format and is suitable for direct injection into animal models.

Titer: >1E+13 VG/mL

Size: 30 µL (Std Pack)

Storage Buffer: Sterile PBS supplemented with final 0.005% Pluronic F-68 and 200 mM NaCl

Infection Protocol:

Vector Map: