AAV2-CAG-mCherry-GFP-LC3

Product Category: Pre-made recombinant AAV | AAV-CAG-mCherry-GFP-LC3 | AAV2-CAG-mCherry-GFP-LC3 | AAV-CBA-mCherry-GFP-LC3 | AAV2-CBA-mCherry-GFP-LC3

Description:
AAV2-CAG-mCherry-GFP-LC3 is a pre-made recombinant AAV (rAAV) vector packaged in serotype 2, containing AAV2 capsid proteins and AAV2 inverted terminal repeats (2× ITRs). This vector expresses the mCherry–GFP–LC3 fusion protein under the control of the CAG (also known as CBA) promoter and serves as a specialized tool for studying autophagy. AAV2 exhibits relatively broad tropism when administered in mice or used in vitro. Early autophagic vesicles have a near-neutral pH (approximately 7.4), under which both GFP and mCherry fluoresce, producing a merged yellow signal. Upon fusion of an autophagosome with a lysosome—a highly acidic organelle with a pH of approximately 4.5–5.0—an autolysosome is formed. Because GFP is acid-labile, its fluorescence is rapidly quenched in acidic conditions, whereas mCherry is acid-stable and remains fluorescent. Consequently, red fluorescent puncta indicate late-stage autophagic vesicles (autolysosomes), while yellow signals represent early-stage autophagic structures. This product is supplied in a ready-to-use format.

Titer: >1E+13 VG/mL

Size: 30 µL (Std Pack)

Storage Buffer: Sterile PBS supplemented with final 0.005% Pluronic F-68 and 200 mM NaCl

Infection Protocol:

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