AAV-Syn-hSNCA-P2A-GFP (Serotype PHP.eB)

Product Category: Pre-made recombinant AAV | AAV-Syn-hSNCA-P2A-GFP | AAV(PHP.eB)-Syn-hSNCA-P2A-GFP

Description:
AAV(PHP.eB)-Syn-hSNCA-P2A-GFP is a pre-made recombinant adeno-associated virus (rAAV) packaged in the PHP.eB capsid (with AAV2 ITRs), designed for efficient neuron-specific co-expression of human alpha-synuclein (hSNCA) and green fluorescent protein (GFP). Expression is driven by the human synapsin (Syn) promoter, enabling selective and robust transgene expression in neuronal populations.

The hSNCA and GFP coding sequences are linked via a self-cleaving P2A peptide, allowing efficient bicistronic expression and near-equimolar production of both proteins from a single transcript. The PHP.eB capsid is engineered for enhanced central nervous system (CNS) transduction following systemic delivery in mice, enabling widespread neuronal expression across the brain and spinal cord.

Human alpha-synuclein, encoded by the SNCA gene, is a key protein implicated in the pathogenesis of Parkinson’s disease and related synucleinopathies. This construct enables simultaneous visualization of transduced cells via GFP while modeling alpha-synuclein overexpression, aggregation, and neurodegenerative processes.

This vector is well-suited for in vivo and in vitro studies of synucleinopathy mechanisms, including protein aggregation, neuronal toxicity, and evaluation of therapeutic strategies.

Ready-to-use format.

Titer: >1E+13 VG/mL

Size: 30 µL (Std Pack)

Storage Buffer: Sterile PBS supplemented with final 0.005% Pluronic F-68 and 200 mM NaCl

Infection Protocol:

Vector Map: