AAV-Syn-GFP-httQ23 (Serotype PHP.eB)

Product Category: Pre-made recombinant AAV | AAV(PHP.eB)-Syn-GFP-httQ23 | AAV-Syn-GFP-httQ23

Description:
AAV(PHP.eB)-Syn-GFP-httQ23 is a pre-made recombinant adeno-associated virus (rAAV) packaged with the engineered PHP.eB capsid (with AAV2 ITRs) designed for neuron-specific expression of a GFP-tagged huntingtin (HTT) protein fragment containing 23 CAG repeats (encoding glutamine, Q), representing the non-pathogenic polyglutamine (polyQ23) tract of the wild-type form. Expression is driven by the human synapsin (Syn) promoter, enabling selective and robust transgene expression in neuronal populations.

The PHP.eB capsid enables enhanced blood–brain barrier (BBB) penetration and widespread neuronal transduction following systemic delivery in mice, making it particularly suitable for CNS-targeted studies. The GFP fusion allows direct visualization of HTT protein localization, intracellular trafficking, and expression patterns specifically in neurons.

As a non-pathogenic control, httQ23 serves as an essential baseline comparator for studies involving mutant huntingtin variants with expanded polyglutamine repeats.

This vector is particularly well-suited for neuroscience research, neuronal protein localization studies, and in vivo CNS modeling. Supplied in a ready-to-use format, AAV(PHP.eB)-Syn-GFP-httQ23 provides high neuronal transduction efficiency and consistent expression in both in vitro neuronal cultures and in vivo mouse models.