AAV-smCAG-NanoLuc (Serotype 8)

Product Category: Pre-made recombinant AAV; AAV-smCAG-NanoLuc; AAV8-smCAG-NanoLuc

Description:
AAV8-smCAG-NanoLuc is a pre-packaged recombinant adeno-associated virus (rAAV) vector utilizing the AAV8 capsid to express NanoLuc luciferase (NanoLuc/Nluc) under the control of the smCAG promoter.

The smCAG promoter is a compact version of the CAG promoter, designed to retain strong and constitutive transcriptional activity while occupying substantially less space within the limited AAV packaging capacity. Its compact size makes smCAG particularly useful for AAV vectors in which preservation of packaging space is important.

NanoLuc luciferase is a small (~19 kDa), ATP-independent luciferase engineered from the deep-sea shrimp Oplophorus gracilirostris. NanoLuc generates a bright bioluminescent signal in the presence of an appropriate substrate and is widely used as a sensitive reporter for quantitative gene-expression and transduction studies.

The AAV8 capsid is a widely used naturally occurring AAV serotype known for its high in vivo transduction efficiency. AAV8 exhibits particularly strong tropism for the liver and can also transduce several other tissues depending on the route of administration, making it a widely used serotype for systemic and tissue-targeted gene delivery studies.

AAV8-smCAG-NanoLuc provides a sensitive bioluminescent reporter system for evaluating AAV8-mediated transduction, biodistribution, and gene delivery in cultured cells and animal models. It can also serve as a positive-control or reference vector for optimizing AAV dose, administration route, and experimental conditions.

Titer: >1E+13 VG/mL

Size: 30 µL (Std Pack)

Reconstitution Buffer: Sterile PBS supplemented with final 0.005% Pluronic F-68 and 200 mM NaCl

Infection Protocol:

Vector Map: