AAV-RAM-d2TTA-TRE-Cre-WPRE (Serotype 1)

Product Category: Pre-made recombinant AAV; Activity-dependent AAV; RAM-d2TTA/TRE AAV; Activity-dependent Cre AAV; AAV1-RAM-d2TTA-TRE-Cre-WPRE

Description:
AAV1-RAM-d2TTA-TRE-Cre-WPRE is a pre-packaged recombinant adeno-associated virus (rAAV) designed for neuronal activity-dependent expression of Cre recombinase. The vector utilizes the RAM (Robust Activity Marking) regulatory system, in which neuronal activity drives expression of the destabilized tetracycline-controlled transactivator d2TTA. In the absence of doxycycline, d2TTA activates the TRE (tetracycline response element) to drive Cre expression.

This activity-dependent transcriptional architecture enables genetic labeling or manipulation of neurons activated during a defined experimental period when combined with compatible Cre-dependent reporters, effectors, or floxed alleles. Cre-mediated recombination provides a lasting genetic mark that persists after activity-dependent Cre expression subsides, while WPRE supports transgene expression.

The vector is packaged in the AAV serotype 1 capsid. It is particularly useful for neuronal ensemble labeling, activity-dependent circuit studies, behavioral neuroscience, and investigations of learning, memory, and experience-dependent neural activation.

The RAM-d2TTA/TRE system operates through a Tet-Off mechanism: doxycycline suppresses TRE-driven Cre expression, while doxycycline withdrawal permits activity-dependent induction. This provides temporal control over the labeling window; restoring doxycycline suppresses further induction but does not reverse completed Cre-mediated recombination.

Size: 100 µL (2 x 50 µL, Std Pack)

Reconstitution Buffer: Sterile PBS supplemented with final 0.005% Pluronic F-68 and 200 mM NaCl

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