AAV-EF1a-GCaMP6s (AAV Serotype 5)

Product Category: Pre-made recombinant AAV; AAV-EF1a-GCaMP6s; AAV5-EF1a-GCaMP6s

Description:
GCaMP is a genetically encoded calcium indicator (GECI) composed of circularly permuted green fluorescent protein (GFP), calmodulin (CaM), and the M13 peptide from myosin light chain kinase. Calcium binding induces a conformational change that increases GFP fluorescence, enabling researchers to monitor intracellular calcium dynamics associated with neuronal activity and other cellular signaling events.

GCaMP6 is a highly sensitive family of calcium indicators available in three variants with distinct response kinetics: GCaMP6f (fast), GCaMP6m (medium), and GCaMP6s (slow). GCaMP6s provides high calcium sensitivity and slower fluorescence decay, making it useful for detecting subtle calcium signals.

AAV5-EF1a-GCaMP6s is a pre-packaged recombinant AAV vector containing an AAV serotype 5 capsid and AAV serotype 2 inverted terminal repeats (ITRs). It expresses GCaMP6s under the elongation factor 1-alpha (EF1a) promoter for fluorescence-based monitoring of intracellular calcium dynamics.

Titer: >1E+13 VG/mL

Size: 100 µL (2 x 50 µL, Std Pack)

Reconstitution Buffer: Sterile PBS supplemented with final 0.005% Pluronic F-68 and 200 mM NaCl

Infection Protocol:

Vector Map: