AAV-EF1a-fDIO-H2B-mCherry (Serotype 2-Retro)

Product Category: Pre-made recombinant AAV; Retrograde AAV; Cre-dependent AAV; Nuclear Reporter AAV; AAV(2-Retro)-EF1α-fDIO-H2B-mCherry-WPRE

Description:
AAV(2-Retro)-EF1α-fDIO-H2B-mCherry-WPRE is a pre-packaged recombinant adeno-associated viral (rAAV) vector using the AAV(2-Retro) capsid and designed for retrograde, Cre-dependent fluorescent labeling of neuronal nuclei.

The vector utilizes the EF1α promoter to drive robust transgene expression. As shown in the plasmid map, the H2B-mCherry cassette is configured in a flip-excision double-floxed inverted orientation (fDIO) using two pairs of incompatible FRT sites. In the appropriate recombinase context, the cassette undergoes recombination to place H2B-mCherry in the expression-competent orientation, providing conditional expression of the fluorescent reporter.

H2B (histone H2B) directs mCherry to chromatin within the nucleus, resulting in strong nuclear-localized red fluorescence rather than diffuse labeling throughout the neuronal cytoplasm and processes. This makes the vector particularly useful for identifying, counting, and tracking the nuclei of retrogradely transduced neuronal populations.

The AAV(2-Retro) capsid is engineered for efficient retrograde neuronal transduction, allowing the vector to be taken up at axon terminals and transported to neuronal cell bodies. The vector also contains WPRE to enhance transgene expression and an hGH poly(A) signal for efficient transcript termination and polyadenylation.

Size: 100 µL (2 x 50 µL, Std Pack)

Reconstitution Buffer: Sterile PBS supplemented with final 0.005% Pluronic F-68 and 200 mM NaCl

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