AAV-EF1α-EGFP-P2A-TAU(P301L) (Serotype 9)

Product Category: Pre-made recombinant AAV | AAV-EF1α-EGFP-P2A-TAU(P301L) | AAV9-EF1α-EGFP-P2A-TAU(P301L)

Description:
AAV9-EF1α-EGFP-P2A-TAU(P301L) is a pre-made recombinant adeno-associated virus packaged in serotype 9 (AAV9 capsid with AAV2 ITRs) designed for robust, ubiquitous expression of enhanced green fluorescent protein (EGFP) and the human tau protein carrying the P301L mutation. Expression is driven by the human EF1α promoter, enabling strong and consistent transgene expression across a broad range of cell types, including neurons and glial cells.

The EGFP and TAU(P301L) are co-expressed from a single transcript via a self-cleaving P2A peptide, allowing efficient production of two separate proteins within the same cell. EGFP provides a convenient fluorescent marker for monitoring transduction efficiency and cellular distribution, while the P301L mutation in the MAPT gene is a well-established pathogenic variant linked to tauopathies such as frontotemporal dementia with parkinsonism linked to chromosome 17 (FTDP-17).

Following expression, mutant tau undergoes abnormal phosphorylation and aggregation, contributing to neurofibrillary tangle formation, synaptic dysfunction, and neurodegeneration. This vector enables simultaneous visualization of infected cells and induction of tau pathology, making it a powerful tool for mechanistic studies.

AAV9-EF1α-EGFP-P2A-TAU(P301L) is ideal for in vivo and in vitro modeling of tau-mediated neurodegeneration, facilitating studies on disease progression, cell-type susceptibility, and evaluation of therapeutic interventions targeting tau aggregation, clearance, or toxicity.

Titer: >1E+13 VG/mL

Size: 30 µL (Std Pack)

Storage Buffer: Sterile PBS supplemented with final 0.005% Pluronic F-68 and 200 mM NaCl

Infection Protocol:

Vector Map: