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AAV-EF1α-DIO-hChR2(H134R)-mCherry (AAV2-Retro)
Product Category: Pre-made recombinant AAV; Cre-OFF optogenetic AAV; Retrograde AAV; AAV(2-Retro)-EF-1α-DIO-hChR2(H134R)-mCherry; AAV2-Retro
Description:
AAV(2-Retro)-EF-1α-DIO-hChR2(H134R)-mCherry is a pre-packaged recombinant AAV using the AAV2-Retro capsid for retrograde neuronal transduction and Cre-regulated optogenetic activation.
The hChR2(H134R)-mCherry cassette is positioned in the transcriptionally active orientation downstream of the EF-1α promoter and flanked by incompatible loxP and lox2272 sites. In the absence of Cre, hChR2(H134R)-mCherry is expressed. In Cre-positive cells, Cre-mediated recombination inverts the cassette and switches transgene expression OFF, making this a Cre-OFF configuration.
hChR2(H134R) is a blue-light-activated cation channel widely used for optogenetic neuronal activation, while mCherry enables visualization of transduced cells. The construct also contains WPRE and an hGH poly(A) signal to support efficient transgene expression.
Combined with the retrograde properties of AAV2-Retro, this vector is useful for projection-specific optogenetic studies in which transgene expression is maintained in Cre-negative neurons and suppressed in Cre-positive neurons.
Titer: >1×10^13 VG/mL
Size: 30 µL (Std Pack)
Reconstitution Buffer: Sterile PBS supplemented with final 0.005% Pluronic F-68 and 200 mM NaCl
Infection Protocol: 
Vector Map:
CITATIONS
| Shipping Weight | 6 lbs |
|---|---|
| Unit Size | 30 µL at >10¹³ VG/mL, 10 µL at >10¹³ VG/mL |
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