AAV-CMV-FLPo (AAV Serotype 8)

Product Category: Pre-made recombinant AAV; AAV-CMV-FLPo; AAV-FLPo; AAV8-CMV-FLPo; AAV8-FLPo

Description: FLP originally identified from Saccharomyces cerevisiae is a site-specific recombinases (SSR) from the Ϝ integrase family and recognizes distinct 34 bp FRT sequence. It has been widely used for generating conditional knockout mice, mediated by FLP/FRT system. Initial use of FLP in mammalian cells revealed inefficient recombinase activity due to thermo-instability of the FLP protein. Subsequent screening for thermo-stable mutants resulted in the identification of FLPe which was 4-fold increase in recombination efficiency at 37 °C. However the recombination efficiency of FLPe in cells remains very low because of its non-mammalian origin.

FLPo is a mouse codon optimized FLPe that greatly increases the FRT recombination efficiency for both in vivo and in vitro in mouse. This AAV8-CMV-FLPo pre-packaged rAAV in serotype 8 (with capsid from AAV serotype 8 and 2xITR from AAV serotype 2) will over-express FLPo recombinase under CMV promoter. Ready to use format.

Titer: > 1×10^13 VG/mL

Size: >30 µL (Std Pack)

Reconstitution Buffer: Sterile PBS supplemented with final 0.005% Pluronic F-68 and 200 mM NaCl

Infection Protocol:

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