AAV-CaMKIIα-GCaMP6s (Serotype 1)

Product Category: Pre-made Recombinant AAV | AAV-CaMKIIα-GCaMP6s | AAV1-CaMKIIα-GCaMP6s | AAV-CaMKII-GCaMP6s | AAV1-CaMKII-GCaMP6s

Description:
GCaMP is a genetically encoded calcium indicator (GECI) engineered from a fusion of green fluorescent protein (GFP), calmodulin (CaM), and the M13 peptide derived from myosin light-chain kinase. Upon binding intracellular Ca²⁺, GCaMP undergoes a calcium-dependent conformational change that increases the proximity and fluorescence efficiency of the GFP moiety, resulting in a robust increase in green fluorescence. This fluorescence change enables real-time monitoring of neuronal activity, action potentials, and receptor-mediated calcium influx in living cells and organisms. A key advantage of GECIs is that they can be genetically targeted to specific cell types, allowing stable, cell-type-specific calcium imaging in vivo.

GCaMP6 represents a next-generation, ultra-sensitive class of GCaMP indicators and significantly outperforms earlier variants in both signal amplitude and detection accuracy of cytosolic Ca²⁺ dynamics. Targeted mutations within the calmodulin domain of GCaMP6 have produced variants with distinct calcium binding and fluorescence decay kinetics, including fast (GCaMP6s), medium (GCaMP6m), and slow (GCaMP6s) response profiles, enabling researchers to select the optimal sensor for their experimental time scale.

AAV1-CaMKII-GCaMP6s is a pre-packaged recombinant AAV vector of serotype 1, containing an AAV1 capsid and AAV2 inverted terminal repeats (ITRs). It drives robust expression of the fast-kinetics calcium indicator GCaMP6s under the control of the CaMKIIα promoter, ensuring neuron-specific expression, particularly in excitatory neurons. This vector is supplied in a ready-to-use format for in vivo and in vitro neuronal calcium imaging applications.

Titer: >1E+13 VG/mL

Size: 30 µL (Std Pack)

Storage Buffer: Sterile PBS supplemented with final 0.005% Pluronic F-68 and 200 mM NaCl

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